LOCUS       sequence-115688-         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Acrylate biosensor. Low copy plasmid that expresses GFP proportional
            to acrylate concentration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-L-acuR
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      113..199
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      291..318
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
       61 gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
      121 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
      181 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
      241 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
      301 cctgacggat ggcctttttg cgtttctaca aactcgctag caagtaaggc cgactgcttc
      361 acaaccgcac tttgatttaa tagaccatac cgtctattat ttctggaaca tttaacttta
      421 agaaggagat atacatatgc gtaaaggtga agaactgttc accggtgttg ttccgatcct
      481 ggttgaactg gacggtgacg ttaacggtca caaattctct gttcgtggtg aaggtgaagg
      541 tgacgctacc aacggtaaac tgaccctgaa attcatctgc accaccggta aactgccggt
      601 tccgtggccg accctggtta ccaccctgac ctacggtgtt cagtgcttcg ctcgttac
//
