LOCUS       sequence-115693-         362 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Acrylate biosensor. Medium copy plasmid that expresses CFP 
            proportional to acrylate concentration. Compatible with all other 
            pJKR plasmids..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM
  TITLE     Synthetic biosensors for precise gene control and real-time 
            monitoring of metabolites.
  JOURNAL   Nucleic Acids Res. 2015 Jul 7. pii: gkv616.
  PUBMED    26152303
REFERENCE   2  (bases 1 to 362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jul 7. pii: gkv616."
COMMENT     Sequence Label: pJKR-O-acuR
FEATURES             Location/Qualifiers
     source          1..362
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     81..109
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     terminator      complement(258..285)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 taaatttaag ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaaa
       61 atttgtgccc attaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaaa
      121 gttcttctcc tttactcata tgtatatctc cttcttaaag ttaaatatgg ccagaaataa
      181 tagacggcat ggtctattaa atcaagtgcg gttgtgaagc gtcggcctta cttgctagca
      241 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc
      301 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca
      361 aa
//
