LOCUS       sequence-115697-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for PITT landing pad knock-in into the Rosa26 
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBDT
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(705..724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(706..724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(746..762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(746..762)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(759..781)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    770..786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(794..800,801..818,819..824))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ggatttttaa ttcagtcagt agtcttaagt ggtctttatt ggcccttcat taaaatctac
       61 tgttcactct aacagaggct gttggtacta gtggcactta agcaacttcc tacggatata
      121 ctagcagatt aagggtcagg gatagaaact agtctagcgt tttgtatacc taccagcttt
      181 atactacctt gttctgatag aaatatttca ggacatctag agtgtactat aaggttgatg
      241 gtaagcttat aaggaacttg aaagtggagt aactactcca tttctctgag gggagaatta
      301 aaatttttga ccaagtgttg ttgagccact gagaatggtc tcagaacata acttcttaag
      361 gaaccttccc agattgccct caacactgca ccacatttgg tcctgcttga acattgccat
      421 ggctcttaaa gtcttaatta agaatattaa ttgtgtaatt attgtttttc ctcctttaga
      481 tcattccttg aggacaggac agtgcttgtt taaggctata tttctgctgt ctgagcagca
      541 acaggtcttc gagatcaaca tgatgttcat aatcccaaga tgttgccatt tatgttctca
      601 gaagcaagca gaggcatgat ggtcagtgac ggcgcgtggc ctccgaggcc attaccctgt
      661 tatccctaca taggcccaat tgccacggcc cggtacccaa ttcgccctat agtgagtcgt
      721 attacgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
      781 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
//
