LOCUS       sequence-11570-e        9404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9404)
  AUTHORS   Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte 
            EM, Voigt CA, Ellington AD
  TITLE     A synthetic genetic edge detection program.
  JOURNAL   Cell. 2009 Jun 26. 137(7):1272-81.
  PUBMED    19563759
REFERENCE   2  (bases 1 to 9404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 26. 137(7):1272-81."
COMMENT     Sequence Label: pEDL3
FEATURES             Location/Qualifiers
     source          1..9404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             106..117
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             138..3182
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGSLSQMWIGDKNQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(140..162)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3217..3288
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3304..3331
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             3479..4189
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     CDS             4190..4222
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     RBS             4262..4273
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4859..4891
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      4939..5010
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5026..5053
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    5068..5086
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5093..5111
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             5130..5141
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      5945..6016
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6032..6059
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    6066..6086
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(6170..6187)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(6449..7399)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      7447..7669
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(8161..8255)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(8285..9076,9077..9145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8908..8927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9146..9250)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9315..9333
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(9337..9380)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    9383..9404
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagagatgat agatcccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      181 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      241 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt
      301 ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata
      361 ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg
      421 tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt
      481 actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt
      541 ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc
      601 aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc
      661 gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat caggatatgt
      721 ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact acacaaatca
      781 gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta ctggaggctg
      841 aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct ttatggcagg
      901 gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg
      961 gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg
     1021 ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga
     1081 ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc
     1141 tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat catcctctgc
     1201 atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca
     1261 actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg
     1321 accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc atggtgccaa
     1381 tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa
     1441 tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag
     1501 gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc
     1561 cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt tgcccgatgt
     1621 acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat
     1681 ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg
     1741 gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac
     1801 agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca
     1861 acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgta
     1921 tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc
     1981 agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc
     2041 tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat ggtaagccgc
     2101 tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc
     2161 ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc gtagtgcaac
     2221 cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag tggcgtctgg
     2281 cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat ctgaccacca
     2341 gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag
     2401 gctctctttc acagatgtgg attggcgata aaaaccaact gctgacgccg ctgcgcgatc
     2461 agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc cgcattgacc
     2521 ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt
     2581 tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct cacgcgtggc
     2641 agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc
     2701 aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg gcgcggattg
     2761 gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga ttagggccgc
     2821 aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat ctgccattgt
     2881 cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg
     2941 aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc agccgctaca
     3001 gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat
     3061 ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg agcccgtcag
     3121 tatcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa
     3181 aagcggccgc tcgagtctaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     3241 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     3301 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct tgcatttaca
     3361 ttttgaaaca tctatagcga taaatgaaac atcttaaaag ttttagtatc atattcgtgt
     3421 tggattattc tgcatttttg gggagaatgg acttactaga gtcacacagg acggccggat
     3481 gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc
     3541 aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat
     3601 ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta
     3661 taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta gcccttcaat
     3721 cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga
     3781 gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg agcttagaac
     3841 ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc
     3901 attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt
     3961 tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg
     4021 catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca
     4081 ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc
     4141 cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga
     4201 cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat cgctactaga
     4261 gaaagaggag aaatactaga tgactataat gataaaaaaa tcggattttt tggcaattcc
     4321 atcggaggag tataaaggta ttctaagtct tcgttatcaa gtgtttaagc aaagacttga
     4381 gtgggactta gttgtagaaa ataaccttga atcagatgag tatgataact caaatgcaga
     4441 atatatttat gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt tattacctac
     4501 aacaggtgat tatatgctga aaagtgtttt tcctgaattg cttggtcaac agagtgctcc
     4561 caaagatcct aatatagtcg aattaagtcg ttttgctgta ggtaaaaata gctcaaagat
     4621 aaataactct gctagtgaaa ttacaatgaa actatttgaa gctatatata aacacgctgt
     4681 tagtcaaggt attacagaat atgtaacagt aacatcaaca gcaatagagc gatttttaaa
     4741 gcgtattaaa gttccttgtc atcgtattgg agacaaagaa attcatgtat taggtgacac
     4801 taaatcggtt gtattgtcta tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc
     4861 tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc
     4921 tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     4981 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     5041 gggcctttct gcgtttatat actagagtcc ctatcagtga tagagattga catccctatc
     5101 agtgatagag atactgagca ctactagaga aagaggagaa atactagatg aaaaacataa
     5161 atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc aataatgata
     5221 ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat ttactcgcga
     5281 tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat taccctaaaa
     5341 aatggaggca atattatgat gacgctaatt taataaaata tgatcctata gtagattatt
     5401 ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct gtaaataaaa
     5461 aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg tttagtttcc
     5521 ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca gaaaaagaca
     5581 actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt gttccttctc
     5641 tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac gatttaacca
     5701 aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa
     5761 aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg caaatgaaac
     5821 tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga gcaattgatt
     5881 gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta ctagagccag
     5941 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     6001 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     6061 ttatatacta gtagcggccg ctgcagtccg gcaaaaaaac gggcaaggtg tcaccaccct
     6121 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg
     6181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6241 tctcgaggtt acattgtcga tctgttcatg gtgaacagct ttaaatgcac caaaaactcg
     6301 taaaagctct gatgtatcta tcttttttac accgttttca tctgtgcata tggacagttt
     6361 tccctttgat atctaacggt gaacagttgt tctacttttg tttgttagtc ttgatgcttc
     6421 actgatagat acaagagcca taagaacctc agatccttcc gtatttagcc agtatgttct
     6481 ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga accattgaga tcatgcttac
     6541 tttgcatgtc actcaaaaat tttgcctcaa aactggtgag ctgaattttt gcagttaaag
     6601 catcgtgtag tgtttttctt agtccgttac gtaggtagga atctgatgta atggttgttg
     6661 gtattttgtc accattcatt tttatctggt tgttctcaag ttcggttacg agatccattt
     6721 gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca
     6781 ccaatttcat attgctgtaa gtgtttaaat ctttacttat tggtttcaaa acccattggt
     6841 taagcctttt aaactcatgg tagttatttt caagcattaa catgaactta aattcatcaa
     6901 ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt tagttctttt aataaccact
     6961 cataaatcct catagagtat ttgttttcaa aagacttaac atgttccaga ttatatttta
     7021 tgaatttttt taactggaaa agataaggca atatctcttc actaaaaact aattctaatt
     7081 tttcgcttga gaacttggca tagtttgtcc actggaaaat ctcaaagcct ttaaccaaag
     7141 gattcctgat ttccacagtt ctcgtcatca gctctctggt tgctttagct aatacaccat
     7201 aagcattttc cctactgatg ttcatcatct gagcgtattg gttataagtg aacgataccg
     7261 tccgttcttt ccttgtaggg ttttcaatcg tggggttgag tagtgccaca cagcataaaa
     7321 ttagcttggt ttcatgctcc gttaagtcat agcgactaat cgctagttca tttgctttga
     7381 aaacaactaa ttcagacata catctcaatt ggtctaggtg attttaatca ctataccaat
     7441 tgagatgggc tagtcaatga taattacatg tccttttcct ttgagttgtg ggtatctgta
     7501 aattctgcta gacctttgct ggaaaacttg taaattctgc tagaccctct gtaaattccg
     7561 ctagaccttt gtgtgttttt tttgtttata ttcaagtggt tataatttat agaataaaga
     7621 aagaataaaa aaagataaaa agaatagatc ccagccctgt gtataactca ctactttagt
     7681 cagttccgca gtattacaaa aggatgtcgc aaacgctgtt tgctcctcta caaaacagac
     7741 cttaaaaccc taaaggctta agtagcaccc tcgcaagctc gggcaaatcg ctgaatattc
     7801 cttttgtctc cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct
     7861 gcgctcacgg ctctggcagt gaatgggggt aaatggcact acaggcgcct tttatggatt
     7921 catgcaagga aactacccat aatacaagaa aagcccgtca cgggcttctc agggcgtttt
     7981 atggcgggtc tgctatgtgg tgctatctga ctttttgctg ttcagcagtt cctgccctct
     8041 gattttccag tctgaccact tcggattatc ccgtgacagg tcattcagac tggctaatgc
     8101 acccagtaag gcagcggtat catcaacagg cttacccgtc ttactgtccc tagtgcttgg
     8161 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     8221 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgtaa acttggtctg
     8281 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8341 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8401 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8461 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8521 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8581 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8641 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8701 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8761 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8821 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8881 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8941 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9001 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9061 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9121 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9181 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9241 gggttccgcg cacatttccc atggtgccac ctgacgtcta agaaaccatt attatcatga
     9301 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag
     9361 ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agag
//
