LOCUS       sequence-115705-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeting vector for PITT landing pad knock-in into the Rosa26 
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBDU
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(705..724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(706..724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggatttttaa ttcagtcagt agtcttaagt ggtctttatt ggcccttcat taaaatctac
       61 tgttcactct aacagaggct gttggtacta gtggcactta agcaacttcc tacggatata
      121 ctagcagatt aagggtcagg gatagaaact agtctagcgt tttgtatacc taccagcttt
      181 atactacctt gttctgatag aaatatttca ggacatctag agtgtactat aaggttgatg
      241 gtaagcttat aaggaacttg aaagtggagt aactactcca tttctctgag gggagaatta
      301 aaatttttga ccaagtgttg ttgagccact gagaatggtc tcagaacata acttcttaag
      361 gaaccttccc agattgccct caacactgca ccacatttgg tcctgcttga acattgccat
      421 ggctcttaaa gtcttaatta agaatattaa ttgtgtaatt attgtttttc ctcctttaga
      481 tcattccttg aggacaggac agtgcttgtt taaggctata tttctgctgt ctgagcagca
      541 acaggtcttc gagatcaaca tgatgttcat aatcccaaga tgttgccatt tatgttctca
      601 gaagcaagca gaggcatgat ggtcagtgac ggcgcgtggc ctccgaggcc attaccctgt
      661 tatccctaca taggcccaat tgccacggcc cggtacccaa ttcgccctat agtgagtcgt
      721 attacgcgcg cttggcgtaa tcatgg
//
