LOCUS       sequence-115707-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses truncated monomeric eGFP and nuclear localized mRuby2 
            (linked with IRES) in a Cre-dependent manner in an AAV backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Chen Y, Saulnier JL, Yellen G, Sabatini BL
  TITLE     A PKA activity sensor for quantitative analysis of endogenous GPCR 
            signaling via 2-photon FRET-FLIM imaging.
  JOURNAL   Front Pharmacol. 2014 Apr 2;5:56. doi: 10.3389/fphar.2014.00056. 
            eCollection 2014.
  PUBMED    24765076
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Pharmacol."; date: "2014-04-2"; volume: "5:56. doi"; pages: " 
            10.3389/fphar.2014.00056. eCollection 2014"
COMMENT     Sequence Label: AAV-FLEX-tmeGFP-IRES-nls-mRuby2
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(467..484)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     694..713
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
       61 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
      121 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctggctgatc attaccagca
      181 gaacacacct atcggagacg ggcccgtcct gctccctgat aatcattatc tgtctactca
      241 gagtaagctc tcaaaagacc caaacgagaa acgggatcac atggtgctgc tcgagttcgt
      301 cactgcctaa cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
      361 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
      421 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
      481 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
      541 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
      601 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
      661 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
      721 gggctgaagg atgcccagaa ggtaccccat tgtatgggat
//
