LOCUS       sequence-115710-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for PhiC31o mRNA synthesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBBK
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      122..131
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt
      121 cgccaccatg gatacctacg ccggagccta cgacagacag agccgggaga gagagaacag
      181 cagcgccgcc agccccgcca cccagagaag cgccaacgag gataaggccg ccgatctgca
      241 gagagaggtg gagagggacg gcggcagatt cagatttgtg ggccacttca gcgaggcccc
      301 tggcaccagc gccttcggca ccgccgagag acccgagttc gagagaatcc tgaacgagtg
      361 tagggccggc aggctgaaca tgatcatcgt gtacgacgtg tcccggttca gcaggctgaa
      421 ggtgatggac gccatcccta tcgtgtccga gctgctggcc ctgggcgtga ccatcgtgtc
      481 cacccaggaa ggcgtcttta gacagggcaa cgtgatggac ctgatccacc tgatcatgag
      541 gctggacgcc agccacaagg agagcagcct gaagagcgcc aagatcctgg acaccaagaa
      601 cctgcagagg gagctgggcg gctatgtggg cggcaaggcc ccctacggct tcgagctggt
      661 gtccgagacc aaggagatca cccggaacgg caggatggtg aacgtggtga tcaacaagct
      721 ggcccacagc accacccccc tgaccggccc cttcgagttt gagcccgacg tgatcaggtg
      781 gtggtggcgg gagatcaaga cc
//
