LOCUS       sequence-115713-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for FLPo mRNA synthesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBBJ
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      110..119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             122..142
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt ggtaccgagc tcggatcccc ccaccggtcg ccaccatggc
      121 tcctaagaag aagaggaagg tgatgagcca gttcgacatc ctgtgcaaga ccccccccaa
      181 ggtgctggtg cggcagttcg tggagagatt cgagaggccc agcggcgaga agatcgccag
      241 ctgtgccgcc gagctgacct acctgtgctg gatgatcacc cacaacggca ccgccatcaa
      301 gagggccacc ttcatgagct acaacaccat catcagcaac agcctgagct tcgacatcgt
      361 gaacaagagc ctgcagttca agtacaagac ccagaaggcc accatcctgg aggccagcct
      421 gaagaagctg atccccgcct gggagttcac catcatccct tacaacggcc agaagcacca
      481 gagcgacatc accgacatcg tgtccagcct gcagctgcag ttcgagagca gcgaggaggc
      541 cgacaagggc aacagccaca gcaagaagat gctgaaggcc ctgctgtccg agggcgagag
      601 catctgggag atcaccgaga agatcctgaa cagcttcgag tacaccagca ggttcaccaa
      661 gaccaagacc ctgtaccagt tcctgttcct ggccacattc atcaactgcg gcaggttcag
      721 cgacatcaag aacgtggacc ccaagagctt caagctggtg cagaacaagt acctgggcgt
      781 gatcattcag tgcctggtga ccgagaccaa gacaagcgtg tccaggcaca tctactt
//
