LOCUS       sequence-115719-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GalT reporter only ( no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: flGalT-SBP-EGFP_puromycin
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..124)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 tacctgcagg tggttcacgt tgaccttgtg ggtgatgctc cagtcgtgca cgaagttgtt
       61 caagttcccc agccagtcct tcaacaacgt gtccacctcg ccaaccagtg gtcttctcgt
      121 ccatggaatt caagctcggt gtcccgatgt ccactgtgat ttgggtatac aatgggtatc
      181 tctgtacatc cagcacctgg taggtgagtg agttcaaacc atcagagagc attgtctcct
      241 ttgtgtgtgc aattcggtca aacctctgag gattgggttc atttttcttg tctcttgagt
      301 ggcggatcat gcgacacctc ccgaccacag catttgggcg agatatagac atgcctctaa
      361 aaactaatct gttaaaaatg tcatcatctt ctcctcccca gccccaataa ttattaggaa
      421 atccattgat ggttagaaac tgttgtttac ttagagcaga gacacctcca aaatactgaa
      481 cataaggtag gctgaatcca aacttatcca ttgcaacgga aatgtgccgt ggctgtgaaa
      541 aacacctgta cgcattatgg tcattcattg gaatgaggtc cacgtcacta aacacaaagc
      601 aggtgtagtc atagtccttc aaggcttctt gaaagccaac attgaggagc ttagcacgat
      661 tgaatatagt gtctcccgcc tggttgataa catagatgcc atagtccagc tgctggcgct
      721 gcaggactgg gtgcaaataa tatagccagt acttgaggtg ctcctgccgg ttgcggaatg
      781 gaatgatgat ggccaccttg tgaggagaga cgcagtccct
//
