LOCUS       sequence-115721-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of amiR-eGFP with a tdTomato gene from the 
            broadly active CAG promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pARA (pKD-G1)
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(230..248)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     498..517
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     692..715
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
       61 attttggcaa agaattggga tccaccggtc gccaccatgg tgagcaaggg cgaggaggtc
      121 atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc
      181 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      241 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac
      301 ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc
      361 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg
      421 acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc
      481 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
      541 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg
      601 aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
      661 caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac
      721 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgg
//
