LOCUS       sequence-115737-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of processed human RegIIIalpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Mukherjee S, Partch CL, Lehotzky RE, Whitham CV, Chu H, Bevins CL, 
            Gardner KH, Hooper LV
  TITLE     Regulation of C-type lectin antimicrobial activity by a flexible 
            N-terminal prosegment.
  JOURNAL   J Biol Chem. 2009 Feb 20;284(8):4881-8. doi: 10.1074/jbc.M808077200.
            Epub 2008 Dec 18.
  PUBMED    19095652
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M808077200"; journalName: "J Biol Chem"; date: 
            "2009-02-20- 20"; volume: "284"; issue: "8"; pages: "4881-8"
COMMENT     Sequence Label: pET3a hRegIIIa
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             444..466
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(497..516)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(498..516)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(600..619)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gttagcagcc ggatcactag tcagtgaact tgcagacata gggtaacctc acattacagt
       61 tataatcttt ccacctcaga aatgctgtgc ttctcgacag gctcgcacag tggccggggc
      121 ttgagatggt ggagggattt ctctcccatg caaagtaatt catcacatca ctgctactcc
      181 actcccaacc ttctccattg ggctcggtgc cctgtgtggg gtcatggagc ccaatccaga
      241 cgtatgagta gctgttacca atgctcttca ccagggagga cacgaaggat ccctcagccc
      301 cactgagcac agacaccagg tttccagagg gccgcttctg gcaggccaga tctgcatctg
      361 tccaggattt tggtgacaaa aacaaggcat agcagtggga gccataggcc ttggagcctt
      421 ttggacatcg aatcatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg
      481 gaaaccgttg tggtctccct atagtgagtc gtattaattt cgcgggatcg agatctcgat
      541 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
      601 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
      661 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
      721 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
      781 cttcctaatg caggagtcgc at
//
