LOCUS       sequence-115741-         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of full length human RegIIIalpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Cash HL, Whitham CV, Hooper LV
  TITLE     Refolding, purification, and characterization of human and murine 
            RegIII proteins expressed in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2006 Jul;48(1):151-9. Epub 2006 Feb 13.
  PUBMED    16504538
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2006-07"; volume: "48(1)"; pages: "151-9. Epub 
            2006 Feb 13"
COMMENT     Sequence Label: pET3a pro-hRegIIIa
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(514..532)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      528..575
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 agatatacat atggaagaac cgcagagaga actgccctct gcacggatcc gctgtcccaa
       61 aggctccaag gcctatggct cccactgcta tgccttgttt ttgtcaccaa aatcctggac
      121 agatgcagat ctggcctgcc agaagcggcc ctctggaaac ctggtgtctg tgctcagtgg
      181 ggctgaggga tccttcgtgt cctccctggt gaagagcatt ggtaacagct actcatacgt
      241 ctggattggg ctccatgacc ccacacaggg caccgagccc aatggagaag gttgggagtg
      301 gagtagcagt gatgtgatga attactttgc atgggagaga aatccctcca ccatctcaag
      361 ccccggccac tgtgcgagcc tgtcgagaag cacagcattt ctgaggtgga aagattataa
      421 ctgtaatgtg aggttaccct atgtctgcaa gttcactgac tagtgatccg gctgctaaca
      481 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
      541 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
      601 atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa
      661 gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac
//
