LOCUS       sequence-115743-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of full length human RegIIIalpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Cash HL, Whitham CV, Hooper LV
  TITLE     Refolding, purification, and characterization of human and murine 
            RegIII proteins expressed in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2006 Jul;48(1):151-9. Epub 2006 Feb 13.
  PUBMED    16504538
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2006-07"; volume: "48(1)"; pages: "151-9. Epub 
            2006 Feb 13"
COMMENT     Sequence Label: pET3a pro-hRegIIIa
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             474..496
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(527..546)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(528..546)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(630..649)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat cactagtcag tgaacttgca gacatagggt aacctcacat tacaggttat
       61 aatctttcca cctcagaaat gctgtgcttc tcgacaggct cgcacagtgg ccggggcttg
      121 agatggtgga gggatttctc tcccatgcaa agtaattcat cacatcactg ctactccact
      181 cccaaccttc tccattgggc tcggtgccct gtgtggggtc atggagccca atccagacgt
      241 atgagtagct gttaccaatg ctcttcacca gggaggacac gaaggatccc tcagccccac
      301 tgagcacaga caccaggttt ccagagggcc gcttctggca ggccagatct gcatctgtcc
      361 aggattttgg tgacaaaaac aaggcatagc agtgggagcc ataggccttg gagcctttgg
      421 gacagcggat ccgtgcagag ggcagttctc tctgcggttc ttccatatgt atatctcctt
      481 cttaaagtta aacaaaatta tttctagagg gaaaccgttg tggtctccct atagtgagtc
      541 gtattaattt cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat
      601 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
      661 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
      721 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
      781 gctcaacggc ctcaacctac tactgggctg cttcctaatg ca
//
