LOCUS       sequence-115745-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of amiR-eGFP from the broadly active CAG 
            promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pARC (pKD-G3)
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     ncRNA           99..125
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           189..230
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(347..366)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    412..467
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(466..485)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
       61 attttggcaa agaattctag tgagtcgacc agtggatcct ggaggcttgc tgaaggctgt
      121 atgctgatga acttcagggt cagcttggtt ttggccactg actgaccaag ctgactgaag
      181 ttcatcagga cacaaggcct gttactagca ctcacatgga acaaatggcc cagatctggc
      241 cgcactcgag atatctagac ccagaattca ctcctcaggt gcaggctgcc tatcagaagg
      301 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
      361 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
      421 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
      481 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
      541 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
      601 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
      661 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
      721 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
      781 tcttatggag atcc
//
