LOCUS       sequence-115747-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of processed mouse RegIIIgamma.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Mukherjee S, Partch CL, Lehotzky RE, Whitham CV, Chu H, Bevins CL, 
            Gardner KH, Hooper LV
  TITLE     Regulation of C-type lectin antimicrobial activity by a flexible 
            N-terminal prosegment.
  JOURNAL   J Biol Chem. 2009 Feb 20;284(8):4881-8. doi: 10.1074/jbc.M808077200.
            Epub 2008 Dec 18.
  PUBMED    19095652
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M808077200"; journalName: "J Biol Chem"; date: 
            "2009-02-20- 20"; volume: "284"; issue: "8"; pages: "4881-8"
COMMENT     Sequence Label: pET3a RegIIIg
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             438..460
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(491..510)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(492..510)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(594..613)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agcagccgga tccctaggcc ttgaatttgc agacataggg taactctaag ttacaataat
       61 tctctctcca cttcagaaat cctgaggctc ttgacagggt accacagtga ttgcctgagg
      121 aagaggaagg attcgtctcc cagttgatgt aattcatcac atcagcattg ctccactccc
      181 atccacctct gttgggttca tagcccagtg tcgggtcatg gagcccaatc catacatatt
      241 ggccactgtt accactgctt ttgatcatgg aggacaggaa ggaagcttca gcgccactga
      301 gcacagacac aagatgtcct gagggcctct tttggcaggc catatctgca tcataccagt
      361 ttttagatac actaaacaag gcatagcaat aggagccata ggcacgggag cccttggggc
      421 agctgctcat atgtatatct ccttcttaaa gttaaacaaa attatttcta gagggaaacc
      481 gttgtggtct ccctatagtg agtcgtatta atttcgcggg atcgagatct cgatcctcta
      541 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
      601 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
      661 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
      721 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
      781 aatgcaggag tcgcat
//
