LOCUS       sequence-115769-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of amiR-eGFP from the broadly active CAG 
            promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pATJ (pKD-G8)
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     ncRNA           100..126
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           190..231
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           238..264
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           328..369
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(486..505)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    551..606
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(605..624)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
       61 cattttggca aagaattcta gtgagtcgac cagtggatcc tggaggcttg ctgaaggctg
      121 tatgctgtgt agttgtactc cagcttgtgt tttggccact gactgacaca agctggtaca
      181 actacacagg acacaaggcc tgttactagc actcacatgg aacaaatggc ccagatcctg
      241 gaggcttgct gaaggctgta tgctgatgaa cttcagggtc agcttggttt tggccactga
      301 ctgaccaagc tgactgaagt tcatcaggac acaaggcctg ttactagcac tcacatggaa
      361 caaatggccc agatctggcc gcactcgaga tatctagacc cagaattcac tcctcaggtg
      421 caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac
      481 tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg
      541 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
      601 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
      661 gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa
      721 agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca tagaa
//
