LOCUS       sequence-115776-         397 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of amiR-eGFP with a tdTomato gene from the 
            broadly active CAG promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pATL (pKD-G10)
FEATURES             Location/Qualifiers
     source          1..397
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      92..101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(231..249)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
       61 cattttggca aagaattggg atccaccggt cgccaccatg gtgagcaagg gcgaggaggt
      121 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
      181 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      241 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
      301 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
      361 ccccgagggc ttcaagtggg agcgcgtgat gaacttc
//
