LOCUS       sequence-115790-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MSCV vector for overexpressing mouse miR-34.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Okada N, Lin CP, Ribeiro MC, Biton A, Lai G, He X, Bu P, Vogel H, 
            Jablons DM, Keller AC, Wilkinson JE, He B, Speed TP, He L
  TITLE     A positive feedback between p53 and miR-34 miRNAs mediates tumor 
            suppression.
  JOURNAL   Genes Dev. 2014 Mar 1;28(5):438-50. doi: 10.1101/gad.233585.113. 
            Epub 2014 Feb 14.
  PUBMED    24532687
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.233585.113"; journalName: "Genes Dev"; date: 
            "2014-03-1- 1"; volume: "28"; issue: "5"; pages: "438-50"
COMMENT     Sequence Label: MSCV-miR-34a
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(143..145,146..148,149..862)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(188..209)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(449..468)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     796..817
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
       61 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
      121 cgatgataat atggccacaa ccatggtgag caagggcgag gagctgttca ccggggtggt
      181 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtctggcga
      241 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
      301 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
      361 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      421 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      481 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      541 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      601 catggccgac aagcagaaga acggcatcaa ggcgaacttc aagatccgcc acaacatcga
      661 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      721 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
      781 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      841 catggacgag ctgtacaagt aatgaattaa ttaagaatta tcaa
//
