LOCUS       sequence-115797-        1094 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and 
            cloning backbone for sgRNA (V2.0).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
  TITLE     Genome engineering using the CRISPR-Cas9 system.
  JOURNAL   Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. 
            Epub 2013 Oct 24.
  PUBMED    24157548
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.143"; journalName: "Nat Protoc"; date: 
            "2013-11"; volume: "8"; issue: "11"; pages: "2281-308"
COMMENT     Sequence Label: pSpCas9n(BB)-2A-Puro (PX462) V2.0
FEATURES             Location/Qualifiers
     source          1..1094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..104)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tcctcgacgt caccgcatgt tagcagactt cctctgccct ctccactgcc gaattccttt
       61 ttcttttttg cctggccggc ctttttcgtg gccgccggcc ttttgtcgcc tcccagctga
      121 gacaggtcga tccgtgtctc gtacaggccg gtgatgctct ggtggatcag ggtggcgtcc
      181 agcacctctt tggtgctggt gtacctcttc cggtcgatgg tggtgtcaaa gtacttgaag
      241 gcggcagggg ctcccagatt ggtcagggta aacaggtgga tgatattctc ggcctgctct
      301 ctgatgggct tatcccggtg cttgttgtag gcggacagca ctttgtccag attagcgtcg
      361 gccaggatca ctctcttgga gaactcgctg atctgctcga tgatctcgtc caggtagtgc
      421 ttgtgctgtt ccacaaacag ctgtttctgc tcattatcct cgggggagcc cttcagcttc
      481 tcatagtggc tggccaggta caggaagttc acatatttgg agggcagggc cagttcgttt
      541 cccttctgca gttcgccggc agaggccagc attctcttcc ggccgttttc cagctcgaac
      601 agggagtact taggcagctt gatgatcagg tcctttttca cttctttgta gcccttggct
      661 tccagaaagt cgatgggatt cttctcgaag ctgcttcttt ccatgatggt gatccccagc
      721 agctctttca cactcttcag tttcttggac ttgccctttt ccactttggc caccaccagc
      781 acagaatagg ccacggtggg gctgtcgaag ccgccgtact tcttagggtc ccagtccttc
      841 tttctggcga tcagcttatc gctgttcctc ttgggcagga tagactcttt gctgaagccg
      901 cctgtctgca cctcggtctt tttcacgata ttcacttggg gcatgctcag cactttccgc
      961 acggtggcaa aatcccggcc cttatcccac acgatctccc cggtttcgcc gtttgtctcg
     1021 atcagaggcc gcttccggat ctcgccgttg gccagggtaa tctcggtctt gaaaaagttc
     1081 atgatgttgc tgta
//
