LOCUS       sequence-115801-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of S. cerevisiae URA3 cloned into pFA6a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Houseley J, Tollervey D
  TITLE     Repeat expansion in the budding yeast ribosomal DNA can occur 
            independently of the canonical homologous recombination machinery.
  JOURNAL   Nucleic Acids Res. 2011 Nov 1;39(20):8778-91. doi: 
            10.1093/nar/gkr589. Epub 2011 Jul 17.
  PUBMED    21768125
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkr589"; journalName: "Nucleic Acids Res"; date: 
            "2011-11-1- 1"; volume: "39"; issue: "20"; pages: "8778-91"
COMMENT     Sequence Label: pFA6a URA3
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(28..50)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     150..169
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        250..268
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     250..267
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        354..570
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
       61 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
      121 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
      181 ccatatggac atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat
      241 acacatacga tttaggtgac actatagaac gcggccgcca gctgaagctt cgtacgctgc
      301 aggtcgacgg atccccgggt taattaaggc gcgccagatc ctccataaag cttttcaatt
      361 catctttttt ttttttgttc ttttttttga ttccggtttc tttgaaattt ttttgattcg
      421 gtaatctccg agcagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata
      481 cgcatatgtg gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga
      541 acaaaaacct gcaggaaacg aagataaatc atgtcgaaag ctacatataa ggaacgtgct
      601 gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga aaagcaaaca
      661 aacttgtgtg cttcattgga tgttcgtacc accaaggaat t
//
