LOCUS       sequence-115839-        7779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For fungal transformation via ATMT. Transformants to be selected by 
            bialaphos resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Sweigard JA, Chumley F, Carroll A, Farrall L, Valent B
  TITLE     A series of vectors for fungal transformation
  JOURNAL   Fungal Genetics Reports, 1997. Vol. 44, Article 19
REFERENCE   2  (bases 1 to 7779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genetics Reports, 1997. Vol. 44, Article 19"
COMMENT     Sequence Label: pFGL822
FEATURES             Location/Qualifiers
     source          1..7779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..45)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(29..45)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             426..977
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     primer_bind     complement(1005..1022)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1005..1021)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1014..1036)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    1224..1248
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2549..3178
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3612..4679
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      4745..4939
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5175..5197)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5283..5423
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5338..5357
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5438..5455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5609..6197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5689..5708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6284..7078)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7503..7527
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    7605..7779
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ctcgagaatt cgagctcggt acccggggga tccactagtt ctagagtcga ggatattgaa
       61 ggagcacttt ttgggcttgg ctggagctag tggaggtcaa caatgaatgc ctattttggt
      121 ttagtcgtcc aggcggtgag cacaaaattt gtgtcgtttg acaagatggt tcatttaggc
      181 aactggtcag atcagcccca cttgtagcag tagcggcggc gctcgaagtg tgactcttat
      241 tagcagacag gaacgaggac attattatca tctgctgctt ggtgcacgat aacttggtgc
      301 gtttgtcaag caaggtaagt gaacgacccg gtcatacctt cttaagttcg cccttcctcc
      361 ctttatttca gattcaatct gacttaccta ttctacccaa gcaacgcttc gattaggaag
      421 taaccatgag cccagaacga cgcccggccg acatccgccg tgccaccgag gcggacatgc
      481 cggcggtctg caccatcgtc aaccactaca tcgagacaag cacggtcaac ttccgtaccg
      541 agccgcagga accgcaggag tggacggacg acctcgtccg tctgcgggag cgctatccct
      601 ggctcgtcgc cgaggtggac ggcgaggtcg ccggcatcgc ctacgcgggt ccctggaagg
      661 cacgcaacgc ctacgactgg acggccgaat cgaccgtgta cgtctccccc cgccaccagc
      721 ggacgggact gggctccacg ctctacaccc acctgctgaa gtccctggag gcacagggct
      781 tcaagagcgt ggtcgctgtc atcgggctgc ccaacgaccc gagcgtgcgc atgcacgagg
      841 cgctcggata tgccccccgc ggcatgctgc gggcggccgg cttcaagcac gggaactggc
      901 atgacgtggg tttctggcag ctggacttca gcctgccggt tccgccccgt ccggtcctgc
      961 ccgtcaccga gatttaggtc gacctgcagg catgcaagct tggcactggc cgtcgtttta
     1021 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     1081 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     1141 cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt cgtttcccgc
     1201 cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag
     1261 agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttggtcca
     1321 tttgtatgtc catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc
     1381 ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac
     1441 atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt
     1501 cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac
     1561 gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac
     1621 caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc
     1681 gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta
     1741 cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac
     1801 ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag
     1861 ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt
     1921 gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag
     1981 gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc
     2041 cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg
     2101 catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc
     2161 aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc
     2221 gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt
     2281 ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc
     2341 ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc
     2401 tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt
     2461 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag
     2521 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg
     2581 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc
     2641 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg
     2701 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa
     2761 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc
     2821 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga
     2881 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg
     2941 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga
     3001 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg
     3061 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg
     3121 cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt
     3181 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc
     3241 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg
     3301 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa
     3361 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc
     3421 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga
     3481 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg
     3541 cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga
     3601 atcggcgtga cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     3661 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     3721 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     3781 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
     3841 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     3901 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     3961 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     4021 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     4081 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     4141 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     4201 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     4261 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     4321 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     4381 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     4441 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     4501 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     4561 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     4621 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     4681 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
     4741 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     4801 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     4861 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     4921 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     4981 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     5041 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     5101 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     5161 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     5221 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     5281 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     5341 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     5401 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     5461 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     5521 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5581 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5641 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5701 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5761 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5821 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5881 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5941 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6001 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     6061 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6121 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6181 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     6241 acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
     6301 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     6361 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     6421 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     6481 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     6541 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     6601 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     6661 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     6721 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
     6781 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
     6841 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
     6901 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
     6961 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
     7021 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
     7081 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
     7141 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
     7201 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
     7261 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
     7321 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
     7381 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
     7441 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
     7501 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
     7561 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
     7621 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
     7681 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt
     7741 cccttatctg ggaactactc acacattatt atggagaaa
//
