LOCUS       sequence-115846-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses gRNA against human CEBPB along with Cas9 with 2A GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: PX458_CEBPB_1
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(270..323)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(339..386)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag
       61 cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
      121 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
      181 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
      241 ccggtgaaca gctcctcgcc cttgctcact gggccaggat tctcctcgac gtcaccgcat
      301 gttagcagac ttcctctgcc ctctccactg ccgaattcct ttttcttttt tgcctggccg
      361 gcctttttcg tggccgccgg ccttttgtcg cctcccagct gagacaggtc gatccgtgtc
      421 tcgtacaggc cggtgatgct ctggtggatc agggtggcgt ccagcacctc tttggtgctg
      481 gtgtacctct tccggtcgat ggtggtgtca aagtacttga aggcggcagg ggctcccaga
      541 ttggtcaggg taaacaggtg gatgatattc tcggcctgct ctctgatggg cttatcccgg
      601 tgcttgttgt aggcggacag cactttgtcc agattagcgt cggccaggat cactctcttg
      661 gagaactcgc tgatctgctc gatgatctcg tccaggtagt gcttgtgctg ttccacaaac
      721 agctgtttct gctcattatc ctcgggggag cccttcagct tctcatagtg gctggccagg
      781 tacaggaagt tcacatattt ggagggcagg gccagttcgt ttcccttctg cagttcgccg
      841 gcagaggcca gcattctctt ccggccgttt tccagctcga acagggagta cttaggcagc
      901 ttgatgatca ggtcc
//
