LOCUS       sequence-115855-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For transfection or retroviral production. Mammalian expression of 
            EGFP driven by the ECMV IRES..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Salat-Canela C, Sese M, Peula C, Ramon y Cajal S, Aasen T
  TITLE     Internal translation of the connexin 43 transcript.
  JOURNAL   Cell Commun Signal. 2014 May 8;12:31. doi: 10.1186/1478-811X-12-31.
  PUBMED    24884945
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Commun
            Signal."; date: "2014-05-8"; volume: "12:31. doi"; pages: " 
            10.1186/1478-811X-12-31"
COMMENT     Sequence Label: pLPCX-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    150..206
                     /label=MCS
                     /note="multiple cloning site"
     promoter        complement(247..450)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(276..296)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        451..754
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gctcgtcaag aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat
       61 ggtggaaaat aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt
      121 aacgttaggg gggggggagg gagaggggcg gatcccgggc ccgcggtacc gtcgactgca
      181 gaattcgaag cttgagctcg agatctgagt ccggtagcgc tagcggatct gacggttcac
      241 taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      301 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
      361 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
      421 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
      481 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
      541 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
      601 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
      661 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
      721 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
      781 taatgacccc gtaattgatt actattacta gagtcggtgg gcctc
//
