LOCUS       sequence-115871-        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NLS-ONL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pONL-N1_3xNLS
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..78
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             82..102
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             106..126
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      151..160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             157..810
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
     CDS             157..810
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagccaccat gctggtacca
       61 aaaaagaaga gaaaggttgt accaaaaaag aagagaaagg ttgtaccaaa aaagaagaga
      121 aaggttttcg aagggccgga tccaccggtc gccaccatgg tgagcgtgat caagcccgag
      181 atgaagatga ggtactacat ggacggctcc gtcaatgggc atgagttcac aatcgagggt
      241 gagggcacag gcagacctta cgagggacat caggagatga cactgcgcgt cacaatggcc
      301 gagggcgggc caatgccttt cgccttcgac ctggtgtccc acgtgttctg ttacggccac
      361 agagttttta ctaaatatcc agaagagatc ccagactatt tcaagcaggc ctttcctgag
      421 ggcctgtcct gggagaggtc cctggagttc gaggacggcg gctccgcctc cgtgagcgcc
      481 cacatcagcc tgaggggcaa caccttctac cacaagtcca agttcaccgg cgtgaacttc
      541 cccgccgacg gccccatcat gcagaaccag agcgtggact gggagccctc caccgagaag
      601 atcaccgcca gcgacggcgt gctgaagggc gacgtgacca tgtacctgaa gctggagggc
      661 ggcggcaacc acaagtgcca gatgaagacc acctacaagg ccgccaagga gatcctggag
      721 atgcccggcg accactacat cggccacagg ctggtgagga agaccgaggg caacatcacc
      781 gagcaggtgg aggacgccgt ggcccactcc ggtaccaagg tgtacgaccc cgagcaacgc
      841 aaacgcatga tcactgggcc tcagtggtgg gctcgctgca agcaaatgaa cgtgctggac
      901 tccttcatca actactatga ttccgagaag cacgccgaga acgccgtgat ttttctgcat
      961 ggtaacgcta cctccagcta cctgtggagg cacgtcgtgc ctcacatcga gcccgtggct
     1021 agatgcatca tccctgatct gatcggaatg ggtaagtccg gca
//
