LOCUS       sequence-115877-        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: GLP1R-Tango
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(1..387,388..1002))
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     916..935
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ctacccaccg tactcgtcaa ttccaagggc atcggtaaac atctgctcaa actcgaagtc
       61 ggccatatcc agagcgccgt agggggcgga gtcgtggggg gtaaatcccg gacccgggga
      121 atccccgtcc cccaacatgt ccagatcgaa atcgtctagc gcgtcggcat gcgccatcgc
      181 cacgtcctcg ccgtctaagt ggagctcgtc ccccaggctg acatcggtcg ggggggccgt
      241 cgacagtctg cgcgtgtgtc ccgcggggag aaaggacagg cgcggagccg ccagccccgc
      301 ctcttcgggg gcgtcgtcgt ccgggagatc gagcaggccc tcgatggtag acccgtaatt
      361 gtttttcgta cgcgcgcggc tgtacgcgga cccactttca catttaagtt gtttttctaa
      421 tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa
      481 taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg tttccctttc
      541 ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa aatgccccac
      601 agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata aaaaggctaa
      661 ttgattttcg agagtttcat actgtttttc tgtaggccgt gtacctaaat gtacttttgc
      721 tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac gtaaaaaatc
      781 ttgccagctt tccccttcta aagggcaaaa gtgagtatgg tgcctatcta acatctcaat
      841 ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc caatacaatg taggctgctc
      901 tacacctagc ttctgggcga gtttacgggt tgttaaacct tcgattccga cctcattaag
      961 cagctctaat gcgctgttaa tcactttact tttatctaat cttagctgga agtacaggtt
     1021 ctcaccggtc gatgaagtgt ccttggccag ggaggagctg gcggtggtgc aggactcatc
     1081 ttg
//
