LOCUS       sequence-115896-        8251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Kopec CD, Li B, Wei W, Boehm J, Malinow R
  TITLE     Glutamate receptor exocytosis and spine enlargement during 
            chemically induced long-term potentiation.
  JOURNAL   J Neurosci. 2006 Feb 15. 26(7):2000-9.
  PUBMED    16481433
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2006 Feb 15. 26(7):2000-9."
COMMENT     Sequence Label: pCI-SEP-GluR2(Q)
FEATURES             Location/Qualifiers
     source          1..8251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     718..742
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1034..1052
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1469..2179
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1496..1524)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2119..2139
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    5365..5486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5376..5395)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5430..5449
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5667..6122
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5754..5773)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5964..5985
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6189..6208)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6308..6330
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6368..6386)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6454..6558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6559..6627,6628..7419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6777..6796)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7590..8178
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8079..8098
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt acttaatacg actcactata ggctagtgga tcccccgggc tgcaggaatt
     1081 cggcacgagg tgcatgggag ggtgctgata ttcccagaca ccaggactac agcggcagct
     1141 cagctaaaaa ctgcattcag ccagtcctcg ggacttcggg agcagggaca ggacgcaagg
     1201 catcaacagc caccagctac aactgggaaa taaggattct tctgccttca cttcgtgttt
     1261 ttagcagctc cttgctaaat atcgacctca caatgcagag gatctaattt gctgaggaaa
     1321 acagtcaaag aaggaagagg aagaaaggga aacgagggga tattttgtgg atgctctact
     1381 tttcttggaa atgcaaaaga ttatgcatat ttctgtcctc ctttctcctg ttttatgggg
     1441 actgattttt ggtgtctctt ctcctaggag taaaggagaa gaacttttca ctggagttgt
     1501 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
     1561 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
     1621 actacctgtt ccttggccaa cacttgtcac tactttaact tatggtgttc aatgcttttc
     1681 aagataccca gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta
     1741 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
     1801 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
     1861 agatggaaac attcttggac acaaattgga atacaactat aacgatcacc aggtgtacat
     1921 catggcagac aaacaaaaga atggaatcaa agctaacttc aaaattagac acaacattga
     1981 agatggaggc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatgggcc
     2041 cgtcctttta ccagacaacc attacctgtt tacaacttct actctttcga aagatcccaa
     2101 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
     2161 catggatgaa ctatacaaag ctagcaacag catacagata ggggggctat ttccaagggg
     2221 cgctgatcaa gaatacagtg catttcgggt agggatggtt cagttttcca cttcggagtt
     2281 cagactgaca ccccatatcg acaatttgga ggtagccaac agtttcgcag tcaccaatgc
     2341 tttctgctcc cagttttcaa gaggagtcta cgcaattttt ggattttatg acaagaagtc
     2401 tgtaaatacc atcacatcat tctgtgggac actccatgtg tccttcatca cacctagctt
     2461 cccaacagat ggcacacatc catttgtcat ccagatgcga cctgacctca aaggagcact
     2521 ccttagcttg attgagtact accaatggga caagttcgca tacctctatg acagtgacag
     2581 aggcttatca acactgcaag ctgttctgga ttctgctgca gagaagaagt ggcaggtgac
     2641 tgctatcaat gtggggaaca tcaacaatga caagaaagat gagacctaca gatcgctctt
     2701 tcaagatctg gagttaaaaa aagaacggcg tgtaatcctg gactgtgaaa gggataaagt
     2761 aaatgacatt gtggaccagg ttattaccat tggaaaacat gttaaagggt accattatat
     2821 cattgcaaat ctgggattca ctgatgggga cctgctgaaa attcagtttg gaggagcaaa
     2881 tgtctctgga tttcagattg tagactacga cgattccctg gtgtctaaat ttatagaaag
     2941 atggtcaaca ctggaagaga aagaataccc tggagcacac acagcgacaa ttaagtatac
     3001 ttcggccctg acgtatgatg ctgtccaagt gatgactgaa gcattccgta accttcggaa
     3061 gcagaggatt gaaatatccc ggagaggaaa tgcaggggat tgtttggcca acccagctgt
     3121 gccctgggga caaggggtcg aaatagaaag ggccctcaag caggttcaag ttgaaggcct
     3181 ctctggaaat ataaagtttg accagaatgg aaaacgaata aactacacaa ttaacatcat
     3241 ggagctcaaa acaaatggac cccggaagat tgggtactgg agtgaagtgg ataaaatggt
     3301 tgtcacccta actgagctcc catcaggaaa tgacacgtct gggcttgaaa acaaaactgt
     3361 ggtggtcacc acaatattgg aatctccata tgttatgatg aagaaaaatc atgaaatgct
     3421 tgaagggaat gagcgttacg agggctactg tgttgactta gctgcagaaa ttgccaaaca
     3481 ctgtgggttc aagtacaagc tgactattgt tggggatggc aagtatgggg ccagggatgc
     3541 cgacaccaaa atttggaatg gtatggttgg agagcttgtc tacgggaaag ctgacattgc
     3601 aattgctcca ttaactatca ctctcgtgag agaagaggtg attgacttct ccaagccctt
     3661 catgagtctt ggaatctcta tcatgatcaa gaagcctcag aagtccaaac caggagtgtt
     3721 ttcctttctt gatcctttag cctatgagat ctggatgtgc attgtgtttg cctacattgg
     3781 ggtcagtgta gttttattcc tggtcagcag atttagcccc tacgagtggc acactgagga
     3841 atttgaagat ggaagagaaa cacaaagtag tgaatcaact aatgaatttg ggatttttaa
     3901 tagtctctgg ttttccttgg gtgcctttat gcagcaggga tgcgatattt cgccaagatc
     3961 cctctctggg cgcattgttg gaggtgtgtg gtggttcttt accctgatca taatctcctc
     4021 ctacacggct aacttagctg ccttcctgac tgtagagagg atggtgtctc ccatcgaaag
     4081 tgctgaggat ctgtctaagc aaacagaaat tgcttatgga acattagact ctggctccac
     4141 taaagagttt ttcaggagat ctaaaatcgc agtgtttgat aaaatgtgga cttatatgag
     4201 gagtgcagag ccctctgtgt ttgtgaggac taccgcagaa ggagtagcca gagtccggaa
     4261 atccaaagga aagtatgcct acttgctgga gtccacaatg aacgagtaca tcgagcagag
     4321 gaagccttgt gacaccatga aagtgggagg aaacttggat tccaaaggct acggcatcgc
     4381 cacacctaaa ggatcctcat taggaaatgc ggttaacctc gcagtactaa aactgaatga
     4441 acaaggcctg ttggacaaat tgaaaaacaa atggtggtac gacaaaggag agtgcggcag
     4501 cgggggaggt gattccaagg aaaagaccag tgccctcagt ctgagcaacg ttgctggagt
     4561 attctacatc cttgtcgggg gccttggttt ggcaatgctg gtggctttga ttgagttctg
     4621 ttacaagtca agggccgagg cgaaacgaat gaaggtggca aagaatccac agaatattaa
     4681 cccatcttcc tcgcagaatt cccagaattt tgcaacttat aaggaaggtt acaacgtata
     4741 tggcatcgag agtgttaaaa tttaggggat gaccttgagt gatgtcatga ggagcaaggc
     4801 aaggctgtca attacaggaa gtactggaga aaatggacgt gttatgactc cagaatttcc
     4861 caaagcagtg catgctgtcc cttacgtgag tcctggcatg ggaatgaatg tcagtgtgac
     4921 tgatctctcg tgattgatag gaaccttctg agtgccttac acaatggttt ccttgtgtgt
     4981 ttattgtcaa agtggtgaga ggcatccgat atcttgaagg cttttctttc agccaagaat
     5041 tcttaactat gtggagttca ccttgaattg taaggaaaga taaattacaa acagagcatc
     5101 attttctact ccgatatcag aggaagcgtg gtggacatgc acagctaaca tggaaatact
     5161 atcatttaac tgaagtcttt gtacagacaa caaacccgtt tccgcagcca ctattgttag
     5221 tctcttgatt cataatgact taagcacact tgacatcaac tgcatcaaga tgtgacctgt
     5281 tttataaaaa aaaaaaaaaa aactcgagaa ttcacgcgtg gtacctctag agtcgacccg
     5341 ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     5401 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     5461 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     5521 ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     5581 tcgataagga tccgggctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     5641 agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg
     5701 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5761 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5821 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5881 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5941 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6001 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6061 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     6121 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     6181 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     6241 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     6301 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     6361 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6421 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6481 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6541 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6601 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6661 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6721 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6781 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6841 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6901 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6961 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7021 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7081 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7141 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7201 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7261 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7321 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7381 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7441 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7501 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7561 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7621 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7681 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7741 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7801 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7861 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7921 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7981 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8041 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8101 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8161 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8221 tggccttttg ctcacatggc tcgacagatc t
//
