LOCUS       sequence-115897-        6210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of mouse Klf4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Takahashi K, Yamanaka S
  TITLE     Induction of pluripotent stem cells from mouse embryonic and adult 
            fibroblast cultures by defined factors.
  JOURNAL   Cell. 2006 Aug 25. 126(4):663-76.
  PUBMED    16904174
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Aug 25. 126(4):663-76."
COMMENT     Sequence Label: pMXs-Klf4
FEATURES             Location/Qualifiers
     source          1..6210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     protein_bind    906..930
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2410..2434)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2485..2859
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(2531..2547)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(2869..3285)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(2885..2901)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2917..2939)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3344..3701
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             3765..4281
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     promoter        4312..4416
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4417..4485,4486..5277)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4635..4654)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5441..6029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5930..5949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6183..6200
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcgtcgacc actgtgctgg cggccgctcg
      781 agtttaaata gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg
      841 gttattatta tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcagctgga
      901 atatcaccac tttgtacaag aaagctgggt cggcgcgccc acccttacat ccactacgtg
      961 ggatttaaaa gtgcctcttc atgtgtaagg caaggtggtc cgacctggaa aaggccctgt
     1021 cgcacttctg gcactgaaag ggccggtgcc ctgtgtgttt gcggtagtgc ctggtcagtt
     1081 catcggagcg ggcgaatttc cacccacagc cgtcccagtc acagtggtaa ggtttctcgc
     1141 ctgtgtgagt tcgcaggtgt gccttgagat gagaactctt ggtataggtt ttgccacagc
     1201 ctgcatagtc acaagtgtgg gtggctgttc ttttccgggg ccacgacctt cttcccctct
     1261 ttggcttggg ctcctctggc aggcaggaac ccggtggcat gagctcttga taatggagag
     1321 aggggacttg tgactgcatc tggtctggca ggaaaggagg gtagttgggc cccggatggg
     1381 gatggaatcc tgggggaaga ggcaggccag ggtgacagtc cctgctgttc agcagttcct
     1441 cgggactcag tgtaggggta gtcctggtgg ggagctgccg ccccaggggg aagtcgtgtg
     1501 tgttgggccg gtggccgttg ctgagctggg gtccagcgct caaatgggcc tctagggacc
     1561 ggctgaccgt gcaggacggg accgcctctt gcttaatctt ggggcacatg cgcggcgggc
     1621 caccgctgta gggcgccact accacggggt ggctgccgtc tgggcttcct ttgctaacac
     1681 tgatgaccga agggctgctg tactcgctgc caggggtggt cagagacgcc ttcagcacaa
     1741 acttgcccat cagcccgcca cctggcggct gaggctgctg tggcggaatg tatactgggt
     1801 ccaactccgg ccgcaggagc tcagccacga agccgcccga ggggctcacg tcattgatgt
     1861 ccgccaggtt gaagggggcc gtgggaggtg gcgcagattc tcggctgtag aggagccctc
     1921 cacctgtgtt gctggcagcc acgcccgggt cacccccggc ccggatcgga tagctgaagc
     1981 tgcaggtgga gggcgcgctg gcagggccgc tgctcgctgg ggaagacgag gatgaagctg
     2041 acgccgaggt ggtcacggtg gcggccaccg attcctggtg ggttagcgag ttggaaagga
     2101 taaagtctag gtccaggagg tcgttgaact cctcggtctc cctccgggct aggagggccg
     2161 ggttgttact gctgcaagct gcaccagctc cgccactctc caggtctgtg gccaccgtcg
     2221 ccgccaggtc gtaggggcgg ccgggaagtg ggggaagtcg cttcatgtga gagagttcct
     2281 cacgccaacg gttagtcggg gcacctgctg gacgcagtgt cttctccctt cccgccgggc
     2341 cggacgcgaa cgtggagaag gacgggagca gagcgtcgct gacagccatg gtgaaggggg
     2401 cggccgcgga gcctgctttt ttgtacaaac ttgtgatttc ccgtaccacc acactgggat
     2461 ccttaattaa ctagctagat ccggcagtct agaggatggt ccacccccgg ggtcggcagc
     2521 cttcacgtgg gcggcgtgta tccaagctgc gatgccgtct actttgaggg cggtgggggt
     2581 ggtcagcagg actgtgtaag gtcctttcca gcgaggttct aggttcttag tctggtgtcg
     2641 gcggacccac actgtgtcgc cgactcggta agggtgaggt accaccggtc ggtccagttg
     2701 ttcttggtag gctgccgcca gaggtctcca gacttcgtgc tggactaagt agagagcctg
     2761 taagtgagct tggagagagg ggctgttagt aactcttgtc atgtcagggt cagggaagtt
     2821 tacaaggggc gggggtgccc catataagat ctcatatggc catatggggg cgcctagaga
     2881 aggagtgagg gctggataaa gggaggatcg aggcggggtc gaacgaggag gttcaagggg
     2941 gagagacggg gcggatggag gaagaggagg cggaggctta gggtgtacaa agggcttgac
     3001 ccagggaggg gggtcaaaag ccaaggcttc ccaggtcacg atgtagggga cctggtctgg
     3061 gtgtccatgc gggccaggtg aaaagacctt gatcttaacc tgggtgatga ggtctcggtt
     3121 aaaggtgccg tctcgcggcc atccgacgtt aaaggttggc cattctgcag agcagaaggt
     3181 aacccaacgt ctcttcttga catctaccga ctggttgtga gcgatccgct cgacatcttt
     3241 ccagtgacct aaggtcaaac ttaagggagt ggtaacagtc tggccctaat tttcagacaa
     3301 atacagaaac acagtcagac agagacaaca cagaacgatg ctgcagcaga caagacgcgc
     3361 ggcgcggctt cggtcccaaa ccgaaagcaa aaattcagac ggaggcggga actgttttag
     3421 gttctcgtct cctaccagaa ccacatatcc ctcctattag gggggtgcac caaagagtcc
     3481 aaaacgatcg ggatttttgg actcaggtcg ggccacaaaa acggcccccg aagtccctgg
     3541 gacgtctccc agggttgcgg ccgggtgttc cgaactcgtc agttccacca cgggtccgcc
     3601 agatacagag ctagttagct aactagtacc gacgcaggcg cataaaatca gtcatagaca
     3661 ctagacaatc ggacagacac agataagttg ctggccagct tacctcccgg tggtgggtcg
     3721 gtggtccctg ggcaggggtc tcccgatccc ggacgagccc ccaaatgaaa gacccccgct
     3781 gacgggtagt caatcactca gaggagaccc tcccaaggaa cagcgagacc acaagtcgga
     3841 tgcaactgca agagggttta ttggatacac gggtacccgg gcgactcagt caatcggagg
     3901 actggcgccc cgagtgaggg gttgtgggct cttttattga gctcggggag cagaagcgcg
     3961 cgaacagaag cgagaagcga actgattggt tagttcaaat aaggcacagg gtcatttcag
     4021 gtccttgggg caccctggaa acatctgatg gttctctaga aactgctgag ggctggaccg
     4081 catctgggga ccatctgttc ttggccctga gccggggcag gaactgctta ccacagatat
     4141 cctgtttggc ccatattcag ctgttccatc tgttcctgac cttgatctga acttttctat
     4201 tctcagttat gtatttttcc atgccttgca aaatggcgtt acttaagcta gcttgccaaa
     4261 cctacaggtg gggtctttca ttatgcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4321 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4381 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4441 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4501 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4561 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4621 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     4681 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4741 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4801 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4861 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4921 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4981 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5041 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5101 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5161 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5221 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5281 tcagaccaag tttactcata tatactttag attgatttgc ggccggccgc aaaaggatct
     5341 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5401 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5461 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5521 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5581 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5641 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5701 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5761 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5821 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5881 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5941 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6001 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6061 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6121 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6181 gcagcgagtc agtgagcgag gaagcggaag
//
