LOCUS       sequence-115917-        3954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast ORF Mak11 in the Gateway entry vector pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: pDONR221 Mak11
FEATURES             Location/Qualifiers
     source          1..3954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1682..1696
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     protein_bind    complement(2079..2178)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2195..2214)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2196..2214)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2219..2235)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2219..2235)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2337..2356)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2348..3157
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2415..2434)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3304..3892
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3793..3812
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctac aaaatgttac tgaatactac ttatactgtc ttcatttttt ttttgcttag
      721 aattataaag ttcaagatca aggccgaaaa ttttttagaa catgaaaaat tgaaaactca
      781 tggtacctca tctcgaaaag atcctaaggg ccaagtcaag aattcgtgta gaaaaatgag
      841 tgctataggc gacaagaacc aatttcgtat cattgtgggc tcatacgagc acaacatttt
      901 gtgtttatcc ttagatatcc caaatcaaaa ggaaaatgat gcagcaaaga caccacattt
      961 tatgccaata tttcattttc aagcccattc gttgagtatt aaatgtttgg ctgtttcaag
     1021 aagataccta gtttctggat ccaatgacga gcacatcaga atttatgatt tgcaaaagag
     1081 gaaggaactg ggtactttgt taagtcatca gggctctatt actgctctac aattctcaca
     1141 tccagcttct tccagtgagg atgctgctgt ttcaaagggg agcaagaata gcaaatggtt
     1201 attatcagca tcagaagacc acaaaattat ggtttggaga gtaaaggact gggaaacagt
     1261 ggggacttta aagggacaca cggcaagggt taatgatgtg gatattcacc ccaccaatag
     1321 aattgccatc agtgttagtg atgaccattc tatacgtctt tggaatttga tgactctaag
     1381 aaatgctgct gtactaaaac taagaaaata taatacaaac gggacttgtg ttagatggtt
     1441 aggcgccaaa ggtgattatt ttgccgtggg tttaagggat agggtgctta tttatgaaac
     1501 tggatcggct aaagttttta aagaaatagt ctttcaaagg aaaactctta tgcatattga
     1561 aacacatatt ttaccattcg ataataagga atacctttct gttggtatca gcgatggtaa
     1621 tgttcatttc tatccttgtg aggagttatt cgaaaaagtt gaagaaaatg aaaagcagga
     1681 agatgatgac gacaaggagg atatcagccc cgctttttct ttattgggtc acacaaaccg
     1741 tattaaagat ttcaagtttt atacgaatga atttggtacc tacctagtca ccattggttc
     1801 tgatggtaaa attgtcgtat gggatatgtc tacaaaagaa caagtagcag tctacgactg
     1861 tggcgaaagg ctaaattgct taacgctatg cgatgaaagc attgagaaat acaacacaat
     1921 gaagaagagg gatgctgaaa cagctgatat aggtgaccaa agtgaggtgg aaagtgatac
     1981 cgaagaatta aagaagataa tgtttggtga gaagaagaaa ctcaataaaa agaagcggaa
     2041 gcaattgaag aagagtaaag tatcagtaga acttgaaaac ccagctttct tgtacaaagt
     2101 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2161 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     2221 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     2281 agataaaata atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     2341 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     2401 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     2461 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     2521 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     2581 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     2641 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     2701 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     2761 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     2821 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     2881 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     2941 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     3001 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     3061 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     3121 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     3181 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     3241 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3301 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3361 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3421 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3481 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3541 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3601 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3661 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3721 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3781 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3841 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3901 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
