LOCUS       sequence-115920-         530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for amiR-eGFP knock-in via landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pAWV
FEATURES             Location/Qualifiers
     source          1..530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           30..56
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           120..161
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           168..194
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           258..299
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 aagggaggta gtgagtcgac cagtggatcc tggaggcttg ctgaaggctg tatgctgatg
       61 aacttcaggg tcagcttggt tttggccact gactgaccaa gctgactgaa gttcatcagg
      121 acacaaggcc tgttactagc actcacatgg aacaaatggc ccagatcctg gaggcttgct
      181 gaaggctgta tgctgtgtag ttgtactcca gcttgtgttt tggccactga ctgacacaag
      241 ctggtacaac tacacaggac acaaggcctg ttactagcac tcacatggaa caaatggccc
      301 agatctggcc gcactcgaga tatctagacc cagctttctt gtacaagtaa agcggccggc
      361 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      421 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      481 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
//
