LOCUS       sequence-115925-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for iCre mRNA synthesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Ohtsuka M, Miura H, Hayashi H, Nakaoka H, Kimura M, Sato M, 
            Gurumurthy CB, Inoko H
  TITLE     Improvement of pronuclear injection-based targeted transgenesis 
            (PITT) by iCre mRNA-mediated site-specific recombination.
  JOURNAL   Transgenic Res. 2013 Aug;22(4):873-5. doi: 
            10.1007/s11248-013-9703-x. Epub 2013 Mar 26.
  PUBMED    23529203
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s11248-013-9703-x"; journalName: "Transgenic Res"; date: 
            "2013-08"; volume: "22"; issue: "4"; pages: "873-5"
COMMENT     Sequence Label: pBBI
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      122..131
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             134..154
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt
      121 cgccaccatg gtgcccaaga agaagaggaa agtctccaac ctgctgactg tgcaccaaaa
      181 cctgcctgcc ctccctgtgg atgccacctc tgatgaagtc aggaagaacc tgatggacat
      241 gttcagggac aggcaggcct tctctgaaca cacctggaag atgctcctgt ctgtgtgcag
      301 atcctgggct gcctggtgca agctgaacaa caggaaatgg ttccctgctg aacctgagga
      361 tgtgagggac tacctcctgt acctgcaagc cagaggcctg gctgtgaaga ccatccaaca
      421 gcacctgggc cagctcaaca tgctgcacag gagatctggc ctgcctcgcc cttctgactc
      481 caatgctgtg tccctggtga tgaggagaat cagaaaggag aatgtggatg ctggggagag
      541 agccaagcag gccctggcct ttgaacgcac tgactttgac caagtcagat ccctgatgga
      601 gaactctgac agatgccagg acatcaggaa cctggccttc ctgggcattg cctacaacac
      661 cctgctgcgc attgccgaaa ttgccagaat cagagtgaag gacatctccc gcaccgatgg
      721 tgggagaatg ctgatccaca ttggcaggac caagaccctg gtgtccacag ctggtgtgga
      781 gaaggcc
//
