LOCUS       sequence-115926-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for Cas9 mRNA synthesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu 
            L, Gurumurthy CB
  TITLE     Mouse Genome Editing Using the CRISPR/Cas System.
  JOURNAL   Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-15.7.27. doi: 
            10.1002/0471142905.hg1507s83.
  PUBMED    25271839
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Hum Genet."; date: "2014-10-1"; volume: "83:15.7.1-15.7.27. doi"; 
            pages: " 10.1002/0471142905.hg1507s83"
COMMENT     Sequence Label: pBGK
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      129..138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             138..203
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             210..230
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt
      121 gcaccggtgc caccatggac tataaggacc acgacggaga ctacaaggat catgatattg
      181 attacaaaga cgatgacgat aagatggccc caaagaagaa gcggaaggtc ggtatccacg
      241 gagtcccagc agccgacaag aagtacagca tcggcctgga catcggcacc aactctgtgg
      301 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca
      361 acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg
      421 aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga
      481 accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct
      541 tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc
      601 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
      661 acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg
      721 ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg
      781 acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaa
//
