LOCUS       sequence-115940-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of PhiC31o recombinase from the broadly active 
            CAG promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBCW
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..70)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 accagccacc accttctgat aggcagcctg cacctgagga gtgaattcac actttccgct
       61 ttttcttagg ggcggccacg tcctcggtgc cgtcctgggc gtcatcctcg tcgtcgtcgg
      121 tgggaggctt ggcccaggtg atgctggctc tcttctcgat gggggtgccc tggcccctgc
      181 cggtggtgct cttggtcacc acgatcttat ccacgaacag gcccacgaac accctcttgt
      241 cgtccacgct ggctctgccc caccaggact tggggccggt ggggtcggcg tcggcatcct
      301 cggggaacca ctgatccagg ggcagcttag gggcctcggc ggcctccagc tcggccagtc
      361 tttcctcggc gccctgctgt ctcagggtca gggcggcctg ctgctttctg aagtgcttcc
      421 tgcccacagg gccgtcatag gctccggcgg ctctgtcctc gtacagctcc tccagggcgt
      481 tcagggcgtc ggctctctcg gccaccaggt tggccctctc gccgctcttc tcgggggcct
      541 cggtcagctt gccgaatctt ctggcggcct cccacagcag ggccagggtc tcctcgtcgc
      601 cctcggcgtg ccggatcttg ttgaagatcc gctcggccac gaacttgtcc agggcggcca
      661 tgctcacatt acaggtgccc tcgtgctggc caggggcgct ggggtccacc acctttcttc
      721 tccggcaccg gtagctgtcc ttgatgctct cctcgcctct cttgctggtc atcacggcgc
      781 cacactcaca gtacagcttg tccatggcgc tcaggatggc ctggcctctg ctca
//
