LOCUS       sequence-115949-         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSC101 based plasmid where pl-tetO expression drives synthesis of 
            construct expressing (N to C terminal) MBP (Maltose Binding 
            Protein), Ntag, and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Sekar K, Gentile AM, Bostick JW, Tyo KE
  TITLE     N-Terminal-Based Targeted, Inducible Protein Degradation in 
            Escherichia coli.
  JOURNAL   PLoS One. 2016 Feb 22;11(2):e0149746. doi: 
            10.1371/journal.pone.0149746. eCollection 2016.
  PUBMED    26900850
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-02-22"; volume: "11(2):e0149746. doi"; pages: " 
            10.1371/journal.pone.0149746. eCollection 2016"
COMMENT     Sequence Label: pKS011
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             322..345
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(491..509)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aacctcgggt cgatcacgtc gctgtataag aaagccggtt cggaaaacct gtattttcaa
       61 tttcataaaa gcggtgcctg gaagctgccg gtgagtctgg tgaaacgtgg tatcgataaa
      121 ctggattata aagaacagct tcaagcatgg cgctgggaac gcgaaattga tgaacgcaac
      181 cgtccgctgt cagatgagga actcgatgca atgtttcctg aaggttacaa ggttttacca
      241 ccgccggccg gctacgtacc gatccgcacg cccgcacaca tggatcgtat cccggcagca
      301 cgcaccactg aaaacagtag cgactacaaa gatgacgatg acaagatggt gagcaagggc
      361 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggttca catggagggc
      421 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      481 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      541 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      601 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcga
//
