LOCUS       sequence-115950-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of amiR-eGFP with a tdTomato gene from the 
            broadly active CAG promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pARA (pKD-G1)
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(177..218)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(282..308)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(454..477)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(652..671)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag tcgcggccgg
      121 ccgctttact tgtacaagaa agctgggtct agatatctcg agtgcggcca gatctgggcc
      181 atttgttcca tgtgagtgct agtaacaggc cttgtgtcct gatgaacttc agtcagcttg
      241 gtcagtcagt ggccaaaacc aagctgaccc tgaagttcat cagcatacag ccttcagcaa
      301 gcctccagga tccactggtc gactcactac ctccctttaa acgatcgacg gccacgaagt
      361 gcttagctta cttgtacagc tcgtccatgc cgtacaggaa caggtggtgg cggccctcgg
      421 agcgctcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccagcttgg
      481 tgtccacgta gtagtagccg ggcagttgca cgggcttctt ggccatgtag atggtcttga
      541 actccaccag gtagtggccg ccgtccttca gcttcagggc ctggtggatc tcgcccttca
      601 gcacgccgtc gcgggggtac aggcgctcgg tggaggcctc ccagcccatg gtcttcttct
      661 gcattacggg gccgtcgggg gggaagttgg tgccgcgcat cttcaccttg tagatcagcg
      721 tgccgtcctg cagggaggag tcctgggtca cggtcaccag accgccgtcc tcgaagttca
      781 tcacgcgctc ccacttgaag ccctcggg
//
