LOCUS       sequence-115953-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of amiR-eGFP with a tdTomato gene from the 
            broadly active CAG promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pARB (pKD-G2)
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(182..223)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(287..313)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(459..482)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(657..676)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tagagtcgcg
      121 gccggccgct ttacttgtac aagaaagctg ggtctagata tctcgagtgc ggccagatct
      181 gggccatttg ttccatgtga gtgctagtaa caggccttgt gtcctgtgta gttgtaccag
      241 cttgtgtcag tcagtggcca aaacacaagc tggagtacaa ctacacagca tacagccttc
      301 agcaagcctc caggatccac tggtcgactc actacctccc tttaaacgat cgacggccac
      361 gaagtgctta gcttacttgt acagctcgtc catgccgtac aggaacaggt ggtggcggcc
      421 ctcggagcgc tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag
      481 cttggtgtcc acgtagtagt agccgggcag ttgcacgggc ttcttggcca tgtagatggt
      541 cttgaactcc accaggtagt ggccgccgtc cttcagcttc agggcctggt ggatctcgcc
      601 cttcagcacg ccgtcgcggg ggtacaggcg ctcggtggag gcctcccagc ccatggtctt
      661 cttctgcatt acggggccgt cgggggggaa gttggtgccg cgcatcttca ccttgtagat
      721 cagcgtgccg tcctgcaggg aggagtcctg ggtcacggtc accagaccgc cgtcctcgaa
      781 gttcatcacg cgctcccact tgaagccctc gg
//
