LOCUS       sequence-115958-         644 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of amiR-eGFP from the broadly active CAG 
            promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pARD (pKD-G4)
FEATURES             Location/Qualifiers
     source          1..644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(81..122)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(186..212)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(261..280)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(342..364)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ccagccacca ccttctgata ggcagcctgc acctgaggag tgaattctgg gtctagatat
       61 ctcgagtgcg gccagatctg ggccatttgt tccatgtgag tgctagtaac aggccttgtg
      121 tcctgtgtag ttgtaccagc ttgtgtcagt cagtggccaa aacacaagct ggagtacaac
      181 tacacagcat acagccttca gcaagcctcc aggatccact ggtcgactca ctagaattct
      241 ttgccaaaat gatgagacag cacaataacc agcacgttgc ccaggagctg taggaaaaag
      301 aagaaggcat gaacatggtt agcagaggct ctagagccgc cggtcacacg ccagaagccg
      361 aaccccgccc tgccccgtcc cccccgaagg cagccgtccc cccgcggaca gccccgaggc
      421 tggagaggga gaaggggacg gcggcgcggc gacgcacgaa ggccctcccc gcccatttcc
      481 ttcctgccgg cgccgcaccg cttcgccccg cgcccgctag agggggtgcg gcggcgcctc
      541 ccagatttcg gctccgcaca gatttgggac aaaggaagtc cctgcgccct ctcgcacgat
      601 taccataaaa ggcaatggct gcggctcgcc gcgcctcgac agcc
//
