LOCUS       sequence-115965-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ManII reporter only (no hook) (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: ManII-SBP-EGFP_puromycin
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(115..136)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     464..483
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     723..744
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agcacactgg atcagttatc tatgcggccg cggatcctta attaattggc cctcgaggcc
       61 ttaaggccgg cccttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
      121 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt
      181 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      241 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac
      301 cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta
      361 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      421 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      481 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
      541 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt
      601 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
      661 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
      721 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcg
//
