LOCUS       sequence-116013-       13330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant 
            expression of Cas9 and multiple cloning sites for insertion of guide
            RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13330)
  TITLE     Sheen lab CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFGC-pcoCas9
FEATURES             Location/Qualifiers
     source          1..13330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(235..487)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(496..543)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(join(544..3207,3397..4833))
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          3208..3396
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             complement(4858..4878)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4885..4932)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     promoter        complement(join(4944..5131,5132..5154,5155..5453))
                     /label=35SPPDK promoter
                     /note="hybrid promoter consisting of the cauliflower mosaic
                     virus 35S enhancer fused to the maize C4PPDK basal promoter
                     (Yoo et al., 2007)"
     primer_bind     complement(5516..5533)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5516..5532)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5525..5547)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5735..5759
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7060..7689
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8118..9191
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9257..9451
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9687..9709)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9795..9935
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9850..9869
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9950..9967)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10121..10709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10201..10220)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10796..11590)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12015..12039
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(12125..12377)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(12387..12938)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(12944..13324)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
ORIGIN
        1 gaattcctcg agtacgtagg atccatttaa attctagagg cgcgccgata tcctctctta
       61 aggtagcgag ctcttaatta atagggataa cagggtaatt aactataacg gtcctaaggt
      121 agcgatggca aacagctatt atgggtatta tgggtggttc tttatgcgga cactgacggc
      181 tttatgcctg caggtcgcga gcgatcgcgg taccgcccgg gcgtcgacag gcctgatcta
      241 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
      301 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
      361 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
      421 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
      481 aacgatctgc agtcacttct tcttcttagc ctgtccagcc ttcttggtag cagctggtct
      541 cttatctcct ccaagctgag aaagatcgat tctggtctcg taaagtccgg tgatagactg
      601 gtggataagg gtagcatcaa gaacctcctt ggtagaggtg tatctttttc tatcgatggt
      661 ggtatcgaag tacttgaaag cagctggagc accaaggttg gtaagggtga aaaggtggat
      721 gatgttctca gcctgctctc tgattggctt atctctgtgc ttgttgtaag cagaaagaac
      781 cttatcaagg ttagcatcag caaggataac tctcttagag aactcagaga tttgctcgat
      841 gatctcatca aggtagtgct tgtgctgctc aacgaaaagc tgcttctgct cgttatcctc
      901 tggagatccc ttcaacttct cgtagtgaga agcaaggtaa aggaagttaa cgtacttaga
      961 tggaagagca agctcgtttc ccttctgaag ctctccagca gaagcaagca ttctctttct
     1021 tccgttctca agctcgaaaa gagagtactt tggcaacttg atgataagat ccttcttaac
     1081 ctccttgtat cccttagcct caaggaaatc gattgggttc ttctcgaaag aagaacgctc
     1141 catgatggtg attccaagaa gctccttaac agacttcaac ttcttagact ttcccttctc
     1201 aaccttagca acaacaagaa cagagtaagc aacggttgga gaatcgaatc ctccgtactt
     1261 ctttgggtcc caatccttct ttctagcgat caacttatca gagtttctct ttggaaggat
     1321 agactcctta gagaatcctc cggtctgaac ctcggttttc ttaacgatgt taacctgtgg
     1381 catagaaaga acctttctaa cggtagcgaa atctcttccc ttatcccaaa cgatctctcc
     1441 ggtctctccg ttggtctcga taagtggtct ctttctgatc tctccgttag caagggtgat
     1501 ctcggtcttg aagaagttca tgatgttaga gtagaagaag tacttagcgg tagcctttcc
     1561 gatctcctgc tcagacttag cgatcatctt tctaacatcg taaaccttgt aatctccgta
     1621 aacgaactca gactccaact ttgggtactt cttgataaga gcggttccaa caacagcgtt
     1681 aaggtaagca tcgtgagcgt ggtggtagtt gttgatctct ctaaccttgt agaactggaa
     1741 atcctttctg aaatcagaaa ccaacttaga cttcaaggtg ataaccttaa cctctctgat
     1801 caacttatcg ttctcatcgt acttggtgtt catacgagaa tcaaggatct gagcaacgtg
     1861 cttggtgatc tgtctggtct caacaagctg tctcttgatg aatccagcct tatcaagctc
     1921 agaaagtcct cctctctcag ccttggtaag gttatcgaac tttctctggg tgatcaactt
     1981 agcgttaaga agctgtctcc agtagttctt catcttctta acaacctcct cagatggaac
     2041 gttatcagac tttcctctgt tcttatcaga acgggtaaga accttgttat cgatagaatc
     2101 atccttcaag aaagactgtg gaacgatgtg atcaacatcg taatcagaaa gtctgttgat
     2161 gtcaagctcc tgatcaacgt acatatctct tccgttctga aggtagtaaa ggtacaactt
     2221 ctcgttctga agctgggtgt tctcaactgg gtgctccttc aagatttgag atccaagctc
     2281 cttgattccc tcctcgattc tcttcattct ctcacgagag ttcttctgtc ccttctgggt
     2341 ggtctggttc tctctagcca tctcgataac gatgttctct ggcttgtgtc tacccataac
     2401 cttaacaagc tcatcaacaa ccttaacggt ctgaaggatt cccttcttga tagctggaga
     2461 tccagcaagg ttagcgatgt gctcgtgaag agaatctccc tgtccagaaa cctgagcctt
     2521 ctggatgtcc tccttgaagg taagagaatc atcgtggata agctgcatga agtttctgtt
     2581 agcgaatcca tcagacttca agaaatcaag gatggtcttt ccagactgct tatctctgat
     2641 tccgttgatc aacttacgag aaagtctacc ccatccggtg tatcttcttc tcttcaactg
     2701 cttcataacc ttatcatcga aaaggtgagc gtaggtcttg agtctctcct cgatcatctc
     2761 tctatcctcg aaaagggtaa gggtaagaac gatgtcctca aggatgtcct cgttctcctc
     2821 gttatcaagg aaatccttat ccttgatgat cttcaaaaga tcgtggtagg ttccaagaga
     2881 agcgttgaat ctatcctcaa ctccagagat ttcaacagaa tcgaagcact cgatcttctt
     2941 gaagtaatcc tccttcaact gcttaacggt aacctttctg ttggtcttga aaagaagatc
     3001 aacgatagcc ttcttctgct ctccagaaag gaaagctggc tttctcattc cctcggtaac
     3061 gtacttaacc ttggtaagct cgttgtaaac ggtgaagtac tcgtaaagaa gagagtgctt
     3121 tggaagaacc ttctcgtttg gaaggttctt atcgaagttg gtcattctct cgatgaaaga
     3181 ctgagcagaa gctcccttat caacaacctg cacatcaaca aattttggtc atatattaga
     3241 aaagttataa attaaaatat acacacttat aaactacaga aaagcaattg ctatatacta
     3301 cattctttta ttttgaaaaa aatatttgaa atattatatt actactaatt aatgataatt
     3361 attatatata tatcaaaggt agaagcagaa acttacctcc tcgaagttcc aaggggtgat
     3421 ggtctcctca gactttctgg tcatccaagc gaaacgagag tttcctctag caagtggtcc
     3481 aacgtagtat gggattctga aggtaaggat cttctcgatc ttctctctgt tatccttcaa
     3541 gaatgggtag aaatcctcct gtctacgaag gatagcgtga agctctccaa ggtggatctg
     3601 gtgtgggata gatccgttat cgaaggttct ctgctttcta agaagatcct ctctgttcaa
     3661 cttaacaaga agctcctcgg ttccatccat cttctcaagg attggcttga tgaacttgta
     3721 gaactcctcc tgagaagctc ctccatcgat gtatccagcg tatccgttct tagactgatc
     3781 gaagaagatt tccttgtact tctctggaag ctgctgtcta acaagagcct tcaaaagggt
     3841 aagatcctgg tggtgctcat cgtatctctt gatcatagaa gcagaaagtg gagccttggt
     3901 gatctcggtg ttaactctaa ggatgtcaga aagaaggata gcatcagaaa ggttcttagc
     3961 agcaaggaaa agatcagcgt actgatctcc gatctgagca agaaggttat caagatcatc
     4021 atcgtaggta tccttagaaa gctgcaactt agcatcctca gcaagatcga agttagactt
     4081 gaagtttggg gtaagtccaa gagaaagagc gataaggttt ccgaaaagtc cgttcttctt
     4141 ctctcctgga agctgagcga taaggttctc aagtctacga gacttagaaa gtctagcaga
     4201 aaggatagcc ttagcatcaa ctccagaagc gttgattggg ttctcctcga aaagctggtt
     4261 gtaggtctga acaagctgga tgaacaactt atcaacatca gagttatctg ggttaaggtc
     4321 tccctcgata aggaagtgtc ctctgaactt gatcatgtga gcaagagcaa ggtagataag
     4381 tctaagatca gccttatcgg tagaatcaac caacttcttt ctaaggtggt agatggttgg
     4441 gtacttctcg tggtaagcaa cctcatcaac gatgtttccg aagattgggt gtctctcgtg
     4501 cttcttatcc tcctcaacaa ggaaagactc ctcaagtctg tggaagaaag aatcatcaac
     4561 cttagccatc tcgttagaga agatttcctg aaggtagcag attctgttct ttcttctggt
     4621 gtatcttctt ctagcggttc tcttcaatct ggtagcctca gcggtctctc cagaatcgaa
     4681 aagaagagca ccgataaggt tcttcttgat agagtgtcta tcggtgtttc caagaacctt
     4741 gaacttctta gatggaacct tgtactcatc ggtgataaca gcccatccaa cagagttggt
     4801 tccgatgtca agtccgatag agtacttctt atcagcagct ggaactccgt ggattccaac
     4861 ctttctcttc ttctttggag ccatcttatc atcatcatcc ttgtaatcct tatcatcatc
     4921 atccttgtaa tccatggatc cacggagcaa ggggagaggg agccgagagc tgcttactac
     4981 gtgaccgccc cgcgcctgct gctgtccgga gctccaacgg agctggagag ggggtgtgtg
     5041 tggcgagaga gtacagcgaa aaggtgagag gcgaacgggt gaatcctgca cgcggaatgc
     5101 ctttattctt ataccatccg tggggcgcga cccaagcttg ggcccaagct tggggcgaag
     5161 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
     5221 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct ccttgtgggt gggggtccat
     5281 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
     5341 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
     5401 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
     5461 tttggtcttc tgagactgta tctttgatat tcttggagta aggcctaagc ttggcactgg
     5521 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     5581 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     5641 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg
     5701 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     5761 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     5821 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     5881 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
     5941 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     6001 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     6061 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     6121 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     6181 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     6241 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     6301 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     6361 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     6421 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     6481 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     6541 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     6601 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     6661 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     6721 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     6781 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
     6841 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
     6901 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
     6961 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
     7021 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
     7081 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
     7141 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
     7201 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
     7261 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
     7321 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
     7381 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
     7441 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
     7501 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
     7561 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
     7621 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
     7681 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     7741 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     7801 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     7861 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
     7921 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     7981 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     8041 ggttggccag gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc
     8101 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg
     8161 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
     8221 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
     8281 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
     8341 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
     8401 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
     8461 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
     8521 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
     8581 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
     8641 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
     8701 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
     8761 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
     8821 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
     8881 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
     8941 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
     9001 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
     9061 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
     9121 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
     9181 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
     9241 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
     9301 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
     9361 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
     9421 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
     9481 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
     9541 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
     9601 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc
     9661 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     9721 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     9781 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     9841 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     9901 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     9961 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    10021 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    10081 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    10141 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    10201 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10261 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    10321 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10381 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10441 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    10501 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    10561 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    10621 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    10681 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    10741 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa
    10801 acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta
    10861 agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc
    10921 agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac
    10981 ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa
    11041 gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg
    11101 gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat
    11161 ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg
    11221 agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat
    11281 cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc
    11341 catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca
    11401 tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt
    11461 ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt
    11521 ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca
    11581 ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc
    11641 taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca
    11701 gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc
    11761 gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc
    11821 taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat
    11881 agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc
    11941 cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc
    12001 tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt
    12061 aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgagctcgg
    12121 atctgataat ttatttgaaa attcataaga aaagcaaacg ttacatgaat tgatgaaaca
    12181 atacaaagac agataaagcc acgcacattt aggatattgg ccgagattac tgaatattga
    12241 gtaagatcac ggaatttctg acaggagcat gtcttcaatt cagcccaaat ggcagttgaa
    12301 atactcaaac cgccccatat gcaggagcgg atcattcatt gtttgtttgg ttgcctttgc
    12361 caacatggga gtccaagatt ctgcagtcaa atctcggtga cgggcaggac cggacggggc
    12421 ggtaccggca ggctgaagtc cagctgccag aaacccacgt catgccagtt cccgtgcttg
    12481 aagccggccg cccgcagcat gccgcggggg gcatatccga gcgcctcgtg catgcgcacg
    12541 ctcgggtcgt tgggcagccc gatgacagcg accacgctct tgaagccctg tgcctccagg
    12601 gacttcagca ggtgggtgta gagcgtggag cccagtcccg tccgctggtg gcggggggag
    12661 acgtacacgg tcgactcggc cgtccagtcg taggcgttgc gtgccttcca ggggcccgcg
    12721 taggcgatgc cggcgacctc gccgtccacc tcggcgacga gccagggata gcgctcccgc
    12781 agacggacga ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg gaagttgacc
    12841 gtgcttgtct cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg
    12901 gcacggcgga tgtcggccgg gcgtcgttct gggctcatcg attcgatttg gtgtatcgag
    12961 attggttatg aaattcagat gctagtgtaa tgtattggta atttgggaag atataatagg
    13021 aagcaaggct atttatccat ttctgaaaag gcgaaatggc gtcaccgcga gcgtcacgcg
    13081 cattccgttc ttgctgtaaa gcgttgtttg gtacactttt gactagcgag gcttggcgtg
    13141 tcagcgtatc tattcaaaag tcgttaatgg ctgcggatca agaaaaagtt ggaatagaaa
    13201 cagaataccc gcgaaattca ggcccggttg ccatgtccta cacgccgaaa taaacgacca
    13261 aattagtaga aaaataaaaa ctgactcgga tacttacgtc acgtcttgcg cactgatttg
    13321 aaaaatctca
//
