LOCUS       sequence-116037-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 10-12 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX4-3
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(384..401)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(384..400)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(393..415)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      464..550
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      642..669
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cccggcaacc ttgggtagca gcgaagtcga ggcatttctg tcctggctgg tctagacgtc
       61 tcggccatgg tcctggcaca gcaccggcaa cagccgctgc accgtttcga gcgcttgctt
      121 gccgccattg ttgctggcga tagccaccac ttggtccggg gtcaggccat ggtcctggca
      181 cagcaccggc aacagccgct gcaccgtttc gagcgcttgc ttgccgccaa tgttgctggc
      241 gatagccacc acttggtccg gggtcaggcc atggtcctgg cacagcaccg gcaacagccg
      301 ctgcaccgtt tcgagcgctt gcttgccgcc aatgttgctg gcgatagcca ccacttggtc
      361 cggggtcagg ggagacgctt aagactggcc gtcgttttac aacgtcgtga ctgggaaaac
      421 atccatgcta gcgttaacgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
      481 tcagtcggaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
      541 taggacaaat ccgccgggag cggatttgaa cgttgtgaag caacggcccg gagggtggcg
      601 ggcaggacgc ccgccataaa ctgccaggca tcaaactaag cagaaggcca tcctgacgga
      661 tggccttttt gcgtttctac aaactcttcc tggctagcgg tacgcgtatt aattgcgttg
      721 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
      781 caac
//
