LOCUS       sequence-116059-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 10-12 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusX4-4
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(393..410)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(393..409)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(402..424)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      473..559
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      651..678
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(837..854)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggtgcgtcac ccggcaacct tgggtagcag cgaagtcgag gcatttctgt cctggctggt
       61 ctagacgtct cggccatggt cctggcacag caccggcaac agccgctgca ccgtttcgag
      121 cgcttgcttg ccgccaccgt tgctggcgat agccaccact tggtccgggg tcaggccatg
      181 gtcctggcac agcaccggca acagccgctg caccgtttcg agcgcttgct tgccgccaat
      241 gttgctggcg atagccacca cttggtccgg ggtcaggcca tggtcctggc acagcaccgg
      301 caacagccgc tgcaccgttt cgagcgcttg cttgccgcca atgttgctgg cgatagccac
      361 cacttggtcc ggggtcaggg gagacgctta agactggccg tcgttttaca acgtcgtgac
      421 tgggaaaaca tccatgctag cgttaacgcg agagtaggga actgccaggc atcaaataaa
      481 acgaaaggct cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
      541 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg
      601 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat
      661 cctgacggat ggcctttttg cgtttctaca aactcttcct ggctagcggt acgcgtatta
      721 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      781 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      841 ctcactgact cgctgcgctc ggtcgttcgg ctgc
//
