LOCUS       sequence-116060-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cytosolic Grx1-roGFP2 (lentiviral vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Gutscher M, Pauleau AL, Marty L, Brach T, Wabnitz GH, Samstag Y, 
            Meyer AJ, Dick TP
  TITLE     Real-time imaging of the intracellular glutathione redox potential.
  JOURNAL   Nat Methods. 2008 Jun;5(6):553-9. doi: 10.1038/nmeth.1212. Epub 2008
            May 11.
  PUBMED    18469822
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1212"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "553-9"
COMMENT     Sequence Label: pEIGW Grx1-roGFP2
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..51
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     36..52
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(161..182)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     510..529
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcggaat tccctcgagg tcgacggtat cgcgataagc
       61 ttgatatcga attcgaagct tgagctcgac tagctagagt cgcggccgcg aattcttact
      121 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
      181 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga gcaggtgctc aggtagtggt
      241 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
      301 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
      361 tctgcttgtc ggccatgata tagacgttgt ggcagttgta gttgtactcc agcttgtgcc
      421 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
      481 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
      541 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
      601 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
      661 gccagggcac gggcagcttg ccggtggtgg agatgaactt ca
//
