LOCUS       sequence-116072-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression plasmid for roGFP2-Grx1; yeast codon optimized 
            roGFP; yeast Grx1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Morgan B, Ezerina D, Amoako TN, Riemer J, Seedorf M, Dick TP
  TITLE     Multiple glutathione disulfide removal pathways mediate cytosolic 
            redox homeostasis.
  JOURNAL   Nat Chem Biol. 2013 Feb;9(2):119-25. doi: 10.1038/nchembio.1142. 
            Epub 2012 Dec 16.
  PUBMED    23242256
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1142"; journalName: "Nat Chem Biol"; date: 
            "2013-02"; volume: "9"; issue: "2"; pages: "119-25"
COMMENT     Sequence Label: p415TEF cyto roGFP2-Grx1(yeast)
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        10..408
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     412..428
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     412..428
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gctggagctc atagcttcaa aatgtttcta ctcctttttt actcttccag attttctcgg
       61 actccgcgca tcgccgtacc acttcaaaac acccaagcac agcatactaa atttcccctc
      121 tttcttcctc tagggtgtcg ttaattaccc gtactaaagg tttggaaaag aaaaaagaga
      181 ccgcctcgtt tctttttctt cgtcgaaaaa ggcaataaaa atttttatca cgtttctttt
      241 tcttgaaaat tttttttttg atttttttct ctttcgatga cctcccattg atatttaagt
      301 taataaacgg tcttcaattt ctcaagtttc agtttcattt ttcttgttct attacaactt
      361 tttttacttc ttgctcatta gaaagaaagc atagcaatct aatctaagtt ttctagaact
      421 agtggatcca ccatggctag cgagttctca aagggtgaag aattgtttac aggtgttgtt
      481 cctattttag tcgaattgga cggtgacgtt aatggtcata agtttagtgt tagtggtgaa
      541 ggtgaaggtg acgcaacata cggtaaattg accttgaagt ttatttcaac tactggtaaa
      601 ttgccagttc cttggccaac tttggtaacc actttaacat atggtgtaca atgtttcagt
      661 agataccctg atcatatgaa acaacacgac tttttcaagt ctgctatgcc agaaggttac
      721 gttcaag
//
