LOCUS       sequence-116079-         544 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes RVD positions 13-14 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):451-63. doi: 10.1089/hum.2015.172
  PUBMED    26854857
REFERENCE   2  (bases 1 to 544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hum.2015"; 
            journalName: "Hum Gene Ther"; date: "2016-06"; volume: "27"; issue: 
            "6"; pages: "451-63"
COMMENT     Sequence Label: pFusB2-4
FEATURES             Location/Qualifiers
     source          1..544
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(324..341)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(324..340)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(333..355)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      404..490
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggtgtgcgtt cacccgggca accttgggta gcagcgaagt cgaggcattt ctgtcctggc
       61 tggtctagac gtctcgatag ccaccacttg gtccggggtc aggccatggt cctggcacag
      121 caccggcaac agccgctgca ccgtttcgag cgcttgcttg ccgccaccgt tgctggcgat
      181 agccaccact tggtccgggg tcaggccatg gtcctggcac agcaccggca acagccgctg
      241 caccgtttcg agcgcttgct tgccgccaat gttgctggcg atagccacca cttggtccgg
      301 ggtcaggcca tgagacgctt aagactggcc gtcgttttac aacgtcgtga ctgggaaaac
      361 atccatgcta gcgttaacgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
      421 tcagtcggaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
      481 taggacaaat ccgccgggag cggatttgaa cgttgtgaag caacggcccg gagggtggcg
      541 ggca
//
