LOCUS       sequence-116110-         814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLAG-HA expression vector with RBPMS carrying mutation R38E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_R38E
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(29..70)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    123..147
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(788..812)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttatctatgc ggccgcggat caggtacgcg tagaatcgag accgaggaga gggttaggga
       61 taggcttacc ttcgaaccgc gggccctcta gacttgagcg gccgccactg tgctggatat
      121 caaccacttt gtacaagaaa gctgggtcta gatatctcga gtgcggccgc tcagcagaac
      181 tgacgggact tccagccctg agaagtagcc tcggagggag ggagccagcg catctgggca
      241 tgcagtgaag cgggataggt gaaagcagga ggaggtagag caggcgctaa ctccgctggg
      301 tacagagggt acggggccca cacttcaggg ctactggggt aaagtgcagg cactgtgagc
      361 tcatatggct ctctggcaat gaactgaggt acagtgttgg gcagaggagt actggggttt
      421 ggagtcccta cgagtttgtt cttggccatc ttcgtgtttg ccttagcaaa ctctagtcgt
      481 agtgtttgcg gaatttcagg atcgaagcgg atgccattca aagcattctt tgcagcctct
      541 gcttctgagc gactgtcaaa actgacaaaa cctacaggct gtttagatgt gagctttata
      601 agagaaccct catagccctt aaatggtctg aaaagcagat agagctcctc aggtttaatg
      661 tccagaggaa ggccactgac aaatagggtc cggacctcct cctcctgaag gttggcctcg
      721 ctcggggtgt tctccttctc ggctttgccg ccgttgttca tgtcgactga attggttccc
      781 atggtggagc ctgctttttt gtacaaactt gttg
//
