LOCUS       sequence-116111-         422 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-HA expression vector with RBPMS2 carrying mutation to introduce
            HindIII site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS2_HindIII
FEATURES             Location/Qualifiers
     source          1..422
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             104..127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             128..154
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    170..194
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atcgcctagg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
       61 cagcctccgg actctagcgt ttaaacttaa gcttggtacc atggactaca aggacgacga
      121 tgacaagtac ccttatgacg tgcccgatta cgctttgaac cagaagctta caagtttgta
      181 caaaaaagca ggcttcatga gcaacctgaa gccggacggc gagcacggcg gcagcaccgg
      241 caccggctcc ggcgcgggct ccggcggcgc cctggaggag gaggtccgga cactgtttgt
      301 cagcggcctc cctgtggaca ttaaacccag agaactctac ttgctcttcc ggccgttcaa
      361 ggggtatgaa gggtccctga tcaagctcac tgcaagacag cctgttggtt ttgtgatctt
      421 tg
//
