LOCUS       sequence-116128-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector: p53 shRNA under control of mouse U6 
            promoter, EGFP-expression driven by CAG promoter to monitor 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Chih B, Engelman H, Scheiffele P
  TITLE     Control of excitatory and inhibitory synapse formation by 
            neuroligins.
  JOURNAL   Science. 2005 Feb 25;307(5713):1324-8. Epub 2005 Jan 27.
  PUBMED    15681343
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2005-02-25"; volume: "307(5713)"; pages: "1324-8. Epub 2005 
            Jan 27"
COMMENT     Sequence Label: pLenLox_U6 p53
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..715
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             2..712
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(41..62)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(302..321)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     649..670
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
       61 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      121 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      181 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
      241 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      301 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      361 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      421 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
      481 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
      541 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      601 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      661 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaggaatt
      721 cgtcgaggga cctaataact tcgtatagca tacatt
//
