LOCUS       sequence-116132-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses RFP-tagged HuC in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  TITLE     Identification of the transcriptome-wide mRNA targets of the 
            neuronal ELAVL proteins (unpublished)
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFRT-TODestRFP_HuC
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..39
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     49..73
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(409..427)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     677..696
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt
       61 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
      121 ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtaccg agctcggatc
      181 catggtgagc aagggcgagg ggtaccatgg tgagcaaggg ggtaccatgg tgagcaaggg
      241 cgggggtacc aggtgagcaa gggcgagggg gtaccatggt gagcaagggc gaggaggtca
      301 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg
      361 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      421 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg
      481 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc
      541 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga
      601 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca
      661 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg
      721 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga
      781 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag a
//
