LOCUS       sequence-116167-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression plasmid for SpCas9 sgRNA (need to clone in spacer 
            into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, 
            Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
  JOURNAL   Nature. 2015 Jun 22. doi: 10.1038/nature14592.
  PUBMED    26098369
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jun 22. doi: 10.1038/nature14592."
COMMENT     Sequence Label: BPK1520
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      223..811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     712..731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
       61 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
      121 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
      181 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
      241 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      301 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
      361 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
      421 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
      481 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
      541 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
      601 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
      661 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      721 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      781 atgctcgtca ggggggcgga gcctatggaa aaa
//
