LOCUS       sequence-116168-         312 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector for S. thermophilus 1 Cas9: 
            CAG-humanSt1Cas9-NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, 
            Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
  JOURNAL   Nature. 2015 Jun 22. doi: 10.1038/nature14592.
  PUBMED    26098369
REFERENCE   2  (bases 1 to 312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jun 22. doi: 10.1038/nature14592."
COMMENT     Sequence Label: MSP1594
FEATURES             Location/Qualifiers
     source          1..312
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcgtgggcgt gggcatcctg aacaaggtga ccggcgagat catccacaan aacagtcgca
       61 tcttccctgc tgctcangct gagaacaacc tggtgcgccg caccaaccgc cagggtcgcc
      121 ggcttgctcg ccgcaagaag caccggcgcg tgcgcctgaa ccgcctgttc gaggagagcg
      181 gcctgatcac cgacttcacc aagatcagca tcaacctgaa cccctaccag ctgcgcgtga
      241 agggcctgac cgacnagctg agcaacnagg agctgttcat cgccctgaan aacatggtga
      301 agcaccgcgg ca
//
