LOCUS       sequence-116169-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human expression vector for S. thermophilus 1 Cas9: 
            CAG-humanSt1Cas9-NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, 
            Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
  JOURNAL   Nature. 2015 Jun 22. doi: 10.1038/nature14592.
  PUBMED    26098369
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Jun 22. doi: 10.1038/nature14592."
COMMENT     Sequence Label: MSP1594
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     77..96
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(185..205)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actattgcaa tgaaaataaa tttcctttat tagccagaag tcagatgctc aaggggcttc
       61 atgatgtccc cataattttt ggcagaggga aaaagatctc agtggtattt gtgagccagg
      121 gcattggcca caccagccac caccttctga taggcagcct gcacctgagg agtgcggccg
      181 ctcacacctt cctcttcttc ttggggtcag ccctgctgaa gtccaacttg ggcttgtcgc
      241 cctcgttctt gatgatgtgc tggttgccca gcacgtcggt gcgcaccttg tagatgctga
      301 tgttgctctt gcccaggccc ttcttgcact ggccgctgtt ggccacgttg cccagcacct
      361 tgatcagggc ctcgccgccc tcgaacttct gcttgtcgta gggcttcagc tccacgtagt
      421 gcttctgctt gggcatggtg cggctcagga agcggaacag ctgttgctcc ttggtctcgg
      481 tgtccttcac cagcagaagg tcgttcttgt acagggtgaa cttgaactcg ctgtcgctgt
      541 ccacgccctc cttcttcttg atgtcgttgt acttctcctg gctgatcttg taggtgcctg
      601 tgcctttctc aaactgcaga tcggcgtact tcagccccag gatctcgtac ttgccggtgg
      661 tcttgttgaa gtacacgtcg gcgcgccagg ggctcacgct ctgcagcacc accttgttgt
      721 tgctgtcctt gggggtgatg tcgatgtggt tgcccagctt gctgtcgtag tacttcaggc
      781 tcttgatctc ggg
//
